Starska Katarzyna, Łukomski Marek, Tchórzewski Henryk, Głowacka Ewa
Katedra Otolaryngologii, Klinika Laryngologii Onkologicznej UM w Łodzi.
Otolaryngol Pol. 2009 Sep;63(7):35-42. doi: 10.1016/S0030-6657(09)70186-6.
Antitumor mechanisms of cellular immune response are connected with the cytokines activity secreted by peripheral blood mononuclear cells (PBMC). The aim of this study was estimation of proinflammatory cytokine (IL-6 and TNF- ) concentration in patients with laryngeal squamous cell carcinoma and analysis of directed influence of neoplasm cells to the function of PBMC and modification of cytokine profile.
For analysis of cytokine secretion, the cultures of isolated peripheral blood mononuclear cells (PBMC) with marginal neoplasm cells and noncancerous adjacent mucosa cells from 55 patients with laryngeal squamous cell carcinoma were established. Production of cytokines in 21h and 72h culture supernatants was detected by Elisa.
Authors reported the increase of IL-6 and decrease of TNF- concentration in cultures of isolated peripheral blood mononuclear cells (PBMC) with marginal neoplasm cells and non-cancerous adjacent mucosa cells.
The presence of laryngeal squamous carcinoma cells in PBMC culture can modify immunocompetent cells activity and production of proinflammatory cytokines.
细胞免疫反应的抗肿瘤机制与外周血单个核细胞（PBMC）分泌的细胞因子活性相关。本研究旨在评估喉鳞状细胞癌患者促炎细胞因子（IL-6和TNF- ）的浓度，并分析肿瘤细胞对PBMC功能的直接影响以及细胞因子谱的改变。
为分析细胞因子分泌情况，建立了55例喉鳞状细胞癌患者的外周血单个核细胞（PBMC）与边缘肿瘤细胞及癌旁非癌黏膜细胞的共培养体系。通过酶联免疫吸附测定法（ELISA）检测21小时和72小时培养上清液中细胞因子的产生情况。
作者报道，在含有边缘肿瘤细胞和癌旁非癌黏膜细胞的外周血单个核细胞（PBMC）共培养体系中，IL-6浓度升高，TNF- 浓度降低。
PBMC培养体系中存在喉鳞状癌细胞可改变免疫活性细胞的活性和促炎细胞因子的产生。
