Eyigor Mete, Eyigor Hulya, Osma Ustun, Yilmaz Mustafa Deniz, Erin Nuray, Selcuk Omer Tarik, Sezer Cem, Gultekin Meral, Koksoy Sadi
Akdeniz University, Medical Faculty, Department of Medical Microbiology, Antalya, Turkey, e-mail:
Iran J Immunol. 2014 Dec;11(4):259-68.
Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation.
To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls.
Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA.
IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05).
Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.
尽管头颈部鳞状细胞癌（HNSCC）中细胞因子失衡已为人熟知，但关于其在发育异常阶段、恶性转化之前的发生情况的数据却很少。
确定喉发育异常患者是否表现出与癌症患者和健康对照不同的细胞因子谱。
对17例新诊断、未治疗的喉鳞状细胞癌（SCC）患者、6例喉发育异常患者以及22例健康对照者的循环细胞因子进行分析。使用流式细胞术Th1/Th2细胞因子阵列试剂盒定量测量白细胞介素-2（IL-2）、IL-4、IL-6、IL-10、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）水平。此外，通过酶联免疫吸附测定法（ELISA）测定IL-8水平。
SCC患者中IL-6、IL-8和IL-10在统计学上显著升高（p<0.05）。SCC患者的IL-8和IL-10水平也高于发育异常患者（p<0.05）。此外，与对照组相比，发育异常患者的IL-6和IL-10均显著升高（p<0.05）。
我们的结果表明，IL-6和IL-10不仅在HNSCC中失衡，在喉发育异常中也失衡。
