非宿主烟草原生质体识别球腔菌 Ecp2 激发子是由一个单一显性基因介导的，该基因与已知的 Cf 基因没有同源性。

Recognition of Cladosporium fulvum Ecp2 elicitor by non-host Nicotiana spp. is mediated by a single dominant gene that is not homologous to known Cf-genes.

机构信息

Laboratory of Plant Breeding, Graduate School of Experimental Plant Sciences, Wageningen University, Wageningen, The Netherlands.

出版信息

Mol Plant Pathol. 2004 Sep 1;5(5):397-408. doi: 10.1111/j.1364-3703.2004.00239.x.

DOI:10.1111/j.1364-3703.2004.00239.x

PMID:20565616

Abstract

SUMMARY Cladosporium fulvum is a fungal pathogen of tomato that grows exclusively in the intercellular spaces of leaves. Ecp2 is one of the elicitor proteins that is secreted by C. fulvum and is specifically recognized by tomato plants containing the resistance gene Cf-Ecp2. Recognition is followed by a hypersensitive response (HR) resulting in resistance. HR-associated recognition of Ecp2 has been observed in Nicotiana paniculata, N. sylvestris, N. tabacum and N. undulata that are non-host plants of C. fulvum. Absence of Ecp2-recognition did not lead to growth of C. fulvum on Nicotiana plants. We show that HR-associated recognition of Ecp2 is mediated by a single dominant gene in N. paniculata. However, based on PCR and hybridization analysis this gene is not homologous to known Cf-genes.

摘要

摘要暗梗孢（Cladosporium fulvum）是番茄的一种真菌病原体，仅在叶片的细胞间隙中生长。Ecp2 是由 C. fulvum 分泌的一种激发子蛋白，被含有抗性基因 Cf-Ecp2 的番茄植株特异性识别。识别后会引发过敏反应（HR），从而产生抗性。在非 C. fulvum 宿主植物的烟草（Nicotiana paniculata）、普通烟草（N. sylvestris）、烟草（N. tabacum）和黄花烟草（N. undulata）中观察到了与 HR 相关的 Ecp2 识别。缺乏 Ecp2 识别并不会导致 C. fulvum 在烟草植株上生长。我们表明，在 N. paniculata 中，HR 相关的 Ecp2 识别是由单个显性基因介导的。然而，基于 PCR 和杂交分析，该基因与已知的 Cf 基因没有同源性。