组成型 PR-1::荧光素酶表达筛选鉴定出对生物亲和性和坏死性病原菌具有不同疾病抗性的拟南芥突变体。
A constitutive PR-1::luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens.
机构信息
Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch, 7701, South Africa.
出版信息
Mol Plant Pathol. 2005 Jan 1;6(1):31-41. doi: 10.1111/j.1364-3703.2004.00261.x.
SUMMARY A complex signal transduction network involving salicylic acid, jasmonic acid and ethylene underlies disease resistance in Arabidopsis. To understand this defence signalling network further, we identified mutants that expressed the marker gene PR-1::luciferase in the absence of pathogen infection. These cir mutants all display constitutive expression of a suite of defence-related genes but exhibit different disease resistance profiles to two biotrophic pathogens, Pseudomonas syringae pv. tomato and Peronospora parasitica NOCO2, and the necrotrophic pathogen Botrytis cinerea. We further characterized cir3, which displays enhanced resistance only to the necrotrophic pathogen. Cir3-mediated resistance to B. cinerea is dependent on accumulated salicylic acid and a functional EIN2 protein.
摘要
在拟南芥中，涉及水杨酸、茉莉酸和乙烯的复杂信号转导网络是其抗病性的基础。为了进一步了解这个防御信号网络，我们鉴定了在没有病原体感染的情况下表达标记基因 PR-1::荧光素酶的突变体。这些 cir 突变体都表现出一套防御相关基因的组成性表达，但对两种生营养型病原体——番茄假单胞菌 pv. 番茄和寄生霜霉病菌 NOCO2 以及坏死型病原体 Botrytis cinerea 的抗病性谱不同。我们进一步对仅对坏死型病原体表现出增强抗性的 cir3 进行了特征描述。Cir3 介导的对 B. cinerea 的抗性依赖于积累的水杨酸和功能正常的 EIN2 蛋白。
Zotero 插件