Institut für Pflanzenbiochemie, Weinberg 3, 06120 Halle/Saale, Germany.
Mol Plant Pathol. 2004 Sep 1;5(5):425-34. doi: 10.1111/j.1364-3703.2004.00242.x.
SUMMARY Jasmonic acid and related oxylipin compounds are plant signalling molecules that are involved in the response to pathogens, insects, wounding and ozone. To explore further the role of jasmonates in stress signal transduction, the response of two jasmonate-signalling mutants, jin1 and jin4, to pathogens and ozone was analysed in this study. Upon treatment with the biotrophic bacterial pathogen Pseudomonas syringae, endogenous jasmonate levels increased in jin1 and jin4 similar to wild-type, demonstrating that these mutants are not defective in jasmonate biosynthesis. Jin1 but not jin4 is more resistant to P. syringae and this higher resistance is accompanied by higher levels of salicylic acid. Jin1 is also more resistant to the necrotrophic fungal pathogen Botrytis cinerea and shows wild-type sensitivity to ozone whereas jin4 is more susceptible to B. cinerea and ozone. These results indicate that the mutations in jin1 and jin4 affect different branches of the jasmonate signalling pathway. Additionally, in this combination of phenotypes, jin1 is unique among all other jasmonate-related mutants described thus far. These data also provide support for a crosstalk between the jasmonate and salicylate pathways.
茉莉酸和相关的氧代脂类化合物是植物信号分子，参与对病原体、昆虫、创伤和臭氧的反应。为了进一步探索茉莉酸在应激信号转导中的作用，本研究分析了两个茉莉酸信号突变体 jin1 和 jin4 对病原体和臭氧的反应。在与生物营养型细菌病原体丁香假单胞菌的处理后，jin1 和 jin4 中内源茉莉酸水平增加，与野生型相似，表明这些突变体在茉莉酸生物合成中没有缺陷。jin1 但不是 jin4 对丁香假单胞菌的抗性更高，这种更高的抗性伴随着更高水平的水杨酸。jin1 对坏死型真菌病原体 Botrytis cinerea 的抗性也更强，对臭氧表现出野生型敏感性，而 jin4 对 Botrytis cinerea 和臭氧的敏感性更高。这些结果表明，jin1 和 jin4 中的突变影响了茉莉酸信号通路的不同分支。此外，在这种表型组合中，jin1 在迄今为止描述的所有其他与茉莉酸相关的突变体中是独特的。这些数据也为茉莉酸和水杨酸途径之间的串扰提供了支持。
