Department of Biology, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei-shi, Tokyo 184-8501, Japan.
Mol Plant Pathol. 2005 Nov 1;6(6):615-27. doi: 10.1111/j.1364-3703.2005.00310.x.
SUMMARY When challenged with the necrotrophic fungal pathogen Alternaria alternata Japanese pear pathotype, all tested ecotypes of Arabidopsis plants failed to show hypersensitive cell death, accumulation of detectable levels of reactive oxygen species or accumulation of phytoalexin. We operationally define A. alternata as a non-host pathogen for Arabidopsis plants and show that the protection against A. alternata demonstrated in this study is a non-host penetration resistance. To characterize non-host penetration resistance, we examined the expression patterns of c. 7000 genes by cDNA microarray analysis in Arabidopsis Col-0 plants after inoculation with A. alternata. After inoculation with A. alternata, the transcript levels of 48 genes increased in Col-0 plants. The expression of genes associated with hypersensitive reaction was induced in the non-host penetration resistance to A. alternata, despite the fact that A. alternata had no visible effect on the plants. The non-host penetration resistance to A. alternata was clearly associated with activation of the jasmonate- and ethylene-signalling pathways. In addition, analysis using histochemical staining of GUS activity suggests that defence reactions in non-host penetration resistance are activated locally. The characterization of non-host pathosystem involving Arabidopsis and A. alternata offers an overview of non-host penetration resistance.
当受到坏死真菌病原体交链格孢日本梨专化型的挑战时，所有测试的拟南芥生态型都未能表现出过敏细胞死亡、可检测水平的活性氧物质积累或植物抗毒素积累。我们将交链格孢定义为拟南芥植物的非寄主病原体，并表明本研究中展示的对交链格孢的保护是一种非寄主穿透抗性。为了表征非寄主穿透抗性，我们通过 cDNA 微阵列分析在拟南芥 Col-0 植物接种交链格孢后检查了约 7000 个基因的表达模式。在接种交链格孢后，Col-0 植物中 48 个基因的转录水平增加。尽管交链格孢对植物没有明显影响，但与过敏反应相关的基因的表达在非寄主穿透抗性中被诱导。拟南芥对交链格孢的非寄主穿透抗性与茉莉酸和乙烯信号通路的激活明显相关。此外，使用 GUS 活性的组织化学染色分析表明，非寄主穿透抗性中的防御反应是局部激活的。涉及拟南芥和交链格孢的非寄主病理系统的表征提供了非寄主穿透抗性的概述。
