拟南芥中 UV-B 诱导子叶张开的染色体基因座。
Chromosomal loci important for cotyledon opening under UV-B in Arabidopsis thaliana.
机构信息
Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a Agricultura, Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad de Buenos Aires, C1417 DSE Buenos Aires, Argentina.
出版信息
BMC Plant Biol. 2010 Jun 16;10:112. doi: 10.1186/1471-2229-10-112.
BACKGROUND
Understanding of the genetic architecture of plant UV-B responses allows extensive targeted testing of candidate genes or regions, along with combinations of those genes, for placement in metabolic or signal transduction pathways.
RESULTS
Composite interval mapping and single-marker analysis methods were used to identify significant loci for cotyledon opening under UV-B in four sets of recombinant inbred lines. In addition, loci important for canalization (stability) of cotyledon opening were detected in two mapping populations. One candidate locus contained the gene HY5. Mutant analysis demonstrated that HY5 was required for UV-B-specific cotyledon opening.
CONCLUSIONS
Structured mapping populations provide key information on the degree of complexity in the genetic control of UV-B-induced cotyledon opening in Arabidopsis. The loci identified using quantitative trait analysis methods are useful for follow-up testing of candidate genes.
背景
对植物 UV-B 响应的遗传结构的理解，允许对候选基因或区域，以及这些基因的组合，进行广泛的靶向测试，以确定它们在代谢或信号转导途径中的位置。
结果
利用复合区间作图和单一标记分析方法，在四组重组自交系中鉴定出 UV-B 下子叶张开的显著位点。此外，在两个作图群体中还检测到了对子叶张开进行 canalization（稳定性）重要的位点。一个候选位点包含 HY5 基因。突变分析表明，HY5 是 UV-B 特异性子叶张开所必需的。
结论
结构映射群体提供了关于拟南芥 UV-B 诱导的子叶张开遗传控制复杂性程度的关键信息。使用数量性状分析方法鉴定的位点，可用于候选基因的后续测试。
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