Grieco M P, Hendricks J D, Scanlan R A, Sinnhuber R O, Pierce D A
J Natl Cancer Inst. 1978 May;60(5):1127-31. doi: 10.1093/jnci/60.5.1127.
Four-week-old rainbow trout (Salmo gairdneri) were fed diets containing 0, 3, 50, 200, 400, and 800 ppm dimethylnitrosamine (DMN) for 52 weeks. At the end of 52 weeks, the fish were fed a control diet without DMN for an additional 26 weeks. Samples were taken at 26, 52, and 78 weeks to determine tumor incidence. A dose-related carcinogenic response was established from these results, and an equation was derived to relate the level of the carcinogen to the hepatocellular carcinoma incidence. From a published dose-response study that used outbred Porton rats, a second equation was derived for comparison. Rats and trout were approximately equal in their sensitivity to DMN carcinogenesis. The median lethal dose after ip injection of DMN was 1,770 mg/kg body weight in rainbow trout. Relative to the range of 15-50 mg/kg body weight reported for several mammalian species, trout were resistant to the acute toxicity of DMN.
给四周大的虹鳟鱼（Salmo gairdneri）投喂含有0、3、50、200、400和800 ppm二甲基亚硝胺（DMN）的饲料，持续52周。在52周结束时，再给这些鱼投喂不含DMN的对照饲料，持续26周。在第26、52和78周采集样本以确定肿瘤发生率。从这些结果中确定了剂量相关的致癌反应，并推导出一个方程，将致癌物水平与肝细胞癌发生率联系起来。从一项已发表的使用远交波特顿大鼠的剂量反应研究中，推导出第二个方程用于比较。大鼠和虹鳟鱼对DMN致癌作用的敏感性大致相同。虹鳟鱼腹腔注射DMN后的半数致死剂量为1770 mg/kg体重。相对于几种哺乳动物物种报告的15 - 50 mg/kg体重范围，虹鳟鱼对DMN的急性毒性具有抗性。
