毛细管电泳法与高效液相色谱法比较研究婴儿苹果汁中棒曲霉素的发生情况。
Comparison between capillary electrophoresis and high performance liquid chromatography for the study of the occurrence of patulin in apple juice intended for infants.
机构信息
Organic and Pharmaceutical Chemistry Department, School of Pharmacy, University of Navarra, 31080 Pamplona, Navarra, Spain.
出版信息
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2429-34. doi: 10.1016/j.fct.2010.06.003. Epub 2010 Jun 8.
Apple juice samples intended for infants purchased in Navarra (Spain) have been analyzed for PAT occurrence. Two capillary electrophoresis methods, based on a MEKC and a CEC system, and an HPLC method were evaluated for the aforementioned study. The CEC system gave less satisfying separations and several practical problems, so samples have been analyzed by MEKC and HPLC. Both methods have been comparable in terms of recovery, precision, limits of detection, volume of organic solvents used and adequate selectivity with regard to PAT and HMF. The analysis time in HPLC has been slightly lower than in the MEKC methodology. The PAT levels obtained in apple juice by both validated methods showed a strong correlation (p<0.001). Therefore, both methodologies are useful for the accurate quantification of patulin in this matrix. The PAT levels obtained in the 20 infant apple juices samples were in a range between <LOD and 29.6 microg L(-1), with a mean concentration of 8.0 microg L(-1) which implies a dietary intake estimation of 104 ng kg(-1)b.w.day(-1) considering a body weight of 10 kg and an apple juice consumption of 130 mL day(-1), 26% of the PMTDI recommended by JECFA.
已对从西班牙纳瓦拉购买的婴儿用苹果汁样品进行了棒曲霉素（PAT）出现情况的分析。为进行上述研究，评估了两种基于 MEKC 和 CEC 系统的毛细管电泳方法以及一种 HPLC 方法。CEC 系统的分离效果较差，并且存在若干实际问题，因此仅使用 MEKC 和 HPLC 对样品进行了分析。两种方法在回收率、精密度、检测限、有机溶剂用量和对 PAT 与 5-羟甲基糠醛（HMF）的足够选择性方面具有可比性。HPLC 分析用时略短于 MEKC 方法。两种经验证的方法在苹果汁中获得的 PAT 水平具有很强的相关性（p<0.001）。因此，这两种方法均适用于对该基质中棒曲霉素的准确定量。在 20 份婴儿苹果汁样品中获得的 PAT 水平在<LOD 至 29.6 μg/L 之间，平均浓度为 8.0 μg/L，这意味着如果考虑体重为 10 kg，每天摄入 130 mL 苹果汁，则每天摄入的 PAT 量为 104 ng/kg.b.w.，占 JECFA 推荐的暂定每日耐受摄入量（PMTDI）的 26%。
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