美国伯基特淋巴瘤细胞系中爱泼斯坦-巴尔病毒DNA的状态

State of Epstein-Barr virus DNA in an American Burkitt's lymphoma line.

作者信息

Koliais S, Bjursell G, Adams A, Lindahl T, Klein G

出版信息

J Natl Cancer Inst. 1978 May;60(5):991-4. doi: 10.1093/jnci/60.5.991.

DOI:10.1093/jnci/60.5.991

PMID:205666

Abstract

A human lymphoma cell line, positive for the Epstein-Barr virus (EBV)-associated nuclear antigen, was recently established from a North American Burkitt's lymphoma. This cell line, SU-AmB-2, contained EBV DNA both in the form of circular, nonintegrated DNA molecules of viral genome length, present in multiple copies per cell, and as integrated sequences. Having DNA present in both of these forms, it resembled cell lines established from African Burkitt's lymphomas. In studies on EBV strain differences, the episomal viral DNA in Burkitt's lymphoma cells may now be compared with viral DNA in nonmalignant cells with the use of cell lines from Burkitt's lymphoma patients of similar geographic origin.

摘要

一种与爱泼斯坦-巴尔病毒（EBV）相关核抗原呈阳性的人类淋巴瘤细胞系，最近从北美伯基特淋巴瘤中建立。该细胞系SU-AmB-2含有EBV DNA，其形式既有病毒基因组长度的环状、非整合DNA分子，每个细胞中有多个拷贝，也有整合序列。由于DNA以这两种形式存在，它类似于从非洲伯基特淋巴瘤中建立的细胞系。在关于EBV毒株差异的研究中，现在可以使用来自地理来源相似的伯基特淋巴瘤患者的细胞系，将伯基特淋巴瘤细胞中的游离病毒DNA与非恶性细胞中的病毒DNA进行比较。