Orthopedics, Spine and Dental Group, RTI Biologics, Inc., Alachua, FL, USA.
J Biomater Appl. 2011 Aug;26(2):151-71. doi: 10.1177/0885328210366061. Epub 2010 Jun 21.
Bone graft substitutes have been developed due to the limited supply and morbidity associated with using autogenous graft material. Allogeneic demineralized bone matrix (DBM) has been used extensively as a clinical graft material because of its inherent osteoinductive and osteoconductive properties. Differential enhancement of these properties may optimize the performance of these products for various orthopedic and craniofacial applications. Commercially available bone paste products consist of formulations that combine DBM with a carrier to facilitate handling and containment. In the present study, we present results of a comprehensive in vitro and in vivo characterization of a 100% human DBM putty product, Puros DBM Putty. Results indicate the DBM particles are completely dispersed in the putty. Data are presented showing the porosity of and cell attachment to Puros DBM Putty, thereby demonstrating the osteoconductive properties of this DBM. Puros DBM Putty was also shown to be osteoinductive in the rat ectopic pouch model. We demonstrate here for the first time that Puros DBM Putty maintains its activity to markedly stimulate or induce bone formation over the entire period of its shelf life. Taken together, these data demonstrate that the 100% human allograft derived Puros DBM Putty could be an effective bone graft substitute.
由于自体移植物材料的供应有限和相关发病率，人们开发了骨移植替代物。同种异体脱矿骨基质（DBM）因其固有的成骨诱导和骨传导特性而被广泛用作临床移植物材料。这些特性的差异增强可能会优化这些产品在各种骨科和颅面应用中的性能。市售的骨糊产品由与载体结合以方便处理和容纳的 DBM 配方组成。在本研究中，我们介绍了 100%人源 DBM 腻子产品 Puros DBM Putty 的全面体外和体内特性研究结果。结果表明 DBM 颗粒完全分散在腻子中。我们提供了 Puros DBM Putty 的孔隙率和细胞附着的数据，从而证明了这种 DBM 的骨传导特性。Puros DBM Putty 还在大鼠异位囊模型中表现出成骨诱导性。我们首次证明，Puros DBM Putty 在整个保质期内保持其活性，可显著刺激或诱导骨形成。综上所述，这些数据表明，100%人源同种异体衍生的 Puros DBM Putty 可能是一种有效的骨移植替代物。
