School of Chemistry and Biochemistry, 901 Atlantic Dr, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
Chem Commun (Camb). 2010 Aug 21;46(31):5686-8. doi: 10.1039/b927313a. Epub 2010 Jun 21.
Substitution of a pyridyl for the hydroxyphenyl moiety in the Green Fluorescent Protein analog p-hydroxybenzylidene-dimethylimidiazolinone produces a chromophore which "turns on" fluorescence in the presence of Zn(2+) or Cd(2+) ions. Such a phenomenon provides "proof of principle" for using GFP chromophores in a variety of sensing applications.
在 Green Fluorescent Protein 类似物 p-羟基苯亚甲基-二甲基恶唑啉酮的羟苯基部分用吡啶基取代会产生一个生色团，该生色团在存在 Zn(2+)或 Cd(2+)离子的情况下“开启”荧光。这种现象为在各种传感应用中使用 GFP 生色团提供了“原理证明”。
