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具有巨大斯托克斯位移的绿色荧光蛋白发色团的激发态锁定氨基类似物。

Excited-state locked amino analogues of the green fluorescent protein chromophore with a giant Stokes shift.

作者信息

Zaitseva Snizhana O, Farkhutdinova Dilara A, Baleeva Nadezhda S, Smirnov Alexander Yu, Zagudaylova Marina B, Shakhov Aleksander M, Astafiev Artyom A, Baranov Mikhail S, Bochenkova Anastasia V

机构信息

Institute of Bioorganic Chemistry, Russian Academy of Sciences Miklukho-Maklaya 16/10 117997 Moscow Russia

Department of Chemistry, Lomonosov Moscow State University Leninskie Gory 1/3 119991 Moscow Russia

出版信息

RSC Adv. 2019 Nov 26;9(66):38730-38734. doi: 10.1039/c9ra08808c. eCollection 2019 Nov 25.

DOI:10.1039/c9ra08808c
PMID:35540244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9076007/
Abstract

We design a novel class of excited-state locked GFP chromophores by introducing an amine group at the double -bond and a difluoroboryl bridge. We show that these chromophores intrinsically exhibit a very large Stokes shift of 1 eV. Further tuning through chemical modifications of their aryl substituents makes them environmentally sensitive.

摘要

我们通过在双键处引入胺基和二氟硼桥，设计了一类新型的激发态锁定绿色荧光蛋白发色团。我们发现这些发色团本质上表现出高达1电子伏特的非常大的斯托克斯位移。通过对其芳基取代基进行化学修饰进一步调节，使它们对环境敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/a6d2eb072991/c9ra08808c-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/52770375e93f/c9ra08808c-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/e912c27f2237/c9ra08808c-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/84874f13b6f6/c9ra08808c-s3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/7e92771e7230/c9ra08808c-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/a6d2eb072991/c9ra08808c-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/52770375e93f/c9ra08808c-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/e912c27f2237/c9ra08808c-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/84874f13b6f6/c9ra08808c-s3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/7e92771e7230/c9ra08808c-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d79/9076007/a6d2eb072991/c9ra08808c-f2.jpg

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Synthesis and properties of geometrical 4-diarylmethylene analogs of the green fluorescent protein chromophore.几何 4-二芳基甲叉基类似物的绿色荧光蛋白发色团的合成与性质。
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Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein.绿色荧光蛋白的本征荧光的起源。
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pH-Sensitive fluorophores from locked GFP chromophores by a non-alternant analogue of the photochemical meta effect.通过光化学间位效应的非交替类似物从锁定的绿色荧光蛋白发色团获得的pH敏感荧光团。
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Fluorescence imaging using synthetic GFP chromophores.使用合成绿色荧光蛋白发色团的荧光成像。
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