Viciana P, Lama C, Pachón J, Rey C, Cisneros J M, Cuello J A
Unidad de Enfermedades Infecciosas (Departamento de Medicina Interna), Hospital Universitario Virgen del Rocío, Sevilla.
Med Clin (Barc). 1991 Mar 30;96(12):445-8.
Adenosine deaminase (ADA) is an essential enzyme for the differentiation and proliferation of T lymphocytes and the monocyte-macrophage system. The basic immunitary response of brucellosis is cellular. To this end, ADA activity was evaluated in brucellosis.
Serum ADA activity was assessed by a colorimetric method in 67 patients with brucellosis, before therapy and at 1, 3 and 6 months of follow up.
Serum ADA activity in brucellosis was higher than that in 52 healthy controls, both in those with the acute febrile noncomplicated form (48 patients) and in those with focal symptoms from one organ (19 patients) (p less than 0.0001 and p less than 0.001). There were no differences between both groups of brucellosis. There was a negative correlation between the duration of the disease and ADA activity. After therapy there was a rapid decrease of ADA values, more marked in patients with noncomplicated brucellosis. During the follow up, only one patient had a new increase in ADA activity, coincident with a clinical and bacteriological relapse, and previous to the increase of IgG anti-Brucella titers.
The results indicate that adenosine deaminase activity is increased during the active stage of brucellosis. It can be considered as a biochemical follow up marker of the disease and, probably, as a marker of relapses.
腺苷脱氨酶（ADA）是T淋巴细胞和单核细胞 - 巨噬细胞系统分化与增殖所必需的酶。布鲁氏菌病的基本免疫反应是细胞免疫反应。为此，对布鲁氏菌病患者的ADA活性进行了评估。
采用比色法对67例布鲁氏菌病患者治疗前及随访1、3和6个月时的血清ADA活性进行评估。
布鲁氏菌病患者血清ADA活性高于52名健康对照者，无论是急性发热无并发症型（48例患者）还是有一个器官局灶症状的患者（19例患者）（p<0.0001和p<0.001）。两组布鲁氏菌病患者之间无差异。疾病持续时间与ADA活性呈负相关。治疗后ADA值迅速下降，在无并发症布鲁氏菌病患者中更为明显。在随访期间，只有1例患者ADA活性重新升高，与临床和细菌学复发同时出现，且在抗布鲁氏菌IgG滴度升高之前。
结果表明，布鲁氏菌病活动期腺苷脱氨酶活性升高。它可被视为该疾病的生化随访标志物，也可能是复发的标志物。
