Institute of Animal Science, University of Bonn, Germany.
Mamm Genome. 2010 Aug;21(7-8):409-18. doi: 10.1007/s00335-010-9269-3. Epub 2010 Jun 22.
The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in pigs. For this purpose, a Duroc x Pietrain F(2) resource population (DUPI) with 319 offspring was used to map QTL for the immune traits blood antibodies and interferon-gamma using 122 microsatellites covering all autosomes. Antibodies response to Mycoplasma hyopneumoniae and tetanus toxoid vaccine and the interferon-gamma (IFNG) serum concentration were measured at three different time points and were used as phenotypes. The differences of antibodies and interferon concentration between different time points were also used for the linkage mapping. Line-cross and imprinting QTL analysis, including two-QTL, were performed using QTL Express. A total of 30 QTL (12, 6, and 12 for mycoplasma, tetanus antibody, and IFNG, respectively) were identified at the 5% chromosome-wide-level significant, of which 28 were detected by line-cross and 2 by imprinting model. In addition, two QTL were identified on chromosome 5 using the two-QTL approach where both loci were in repulsion phase. Most QTL were detected on pig chromosomes 2, 5, 11, and 18. Antibodies were increased over time and immune traits were found to be affected by sex, litter size, parity, and month of birth. The results demonstrated that antibody and IFNG concentration are influenced by multiple chromosomal areas. The flanking markers of the QTL identified for IFNG on SSC5 did incorporate the position of the porcine IFNG gene. The detected QTL will allow further research in these QTL regions for candidate genes and their utilization in selection to improve the immune response and disease resistance in pig.
本研究旨在检测猪的先天和适应性免疫的数量性状位点（QTL）。为此，使用杜洛克×皮特兰 F2 资源群体（DUPI），该群体有 319 个后代，使用覆盖所有常染色体的 122 个微卫星，对血液抗体和干扰素-γ的免疫性状进行 QTL 作图。在三个不同时间点测量对支原体和破伤风类毒素疫苗的抗体反应和干扰素-γ（IFNG）血清浓度，并将其用作表型。还将不同时间点的抗体和干扰素浓度差异用于连锁作图。使用 QTL Express 进行线交和印迹 QTL 分析，包括双 QTL 分析。在 5%染色体-wide 水平显著的情况下，共鉴定出 30 个 QTL（分别为支原体、破伤风抗体和 IFNG 的 12、6 和 12 个），其中 28 个由线交检测，2 个由印迹模型检测。此外，使用双 QTL 方法在第 5 号染色体上鉴定出两个 QTL，两个位点都处于相斥阶段。大多数 QTL 都在猪的第 2、5、11 和 18 号染色体上检测到。抗体随时间增加，免疫性状受性别、窝仔数、胎次和出生月份的影响。结果表明，抗体和 IFNG 浓度受多个染色体区域的影响。在 SSC5 上鉴定出的 IFNG 的侧翼标记确实包含了猪 IFNG 基因的位置。检测到的 QTL 将允许在这些 QTL 区域进一步研究候选基因，并将其用于选择，以提高猪的免疫反应和抗病性。
