Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
J Econ Entomol. 2010 Jun;103(3):1012-8. doi: 10.1603/ec09012.
Starch gel electrophoresis of isozymes was used to estimate gene flow among nine populations of Stomoxys calcitrans (L.) (Diptera: Muscidae) from Thailand. Of the 13 putative loci, nine polymorphic loci were detected. Limited genetic differentiation among populations was observed (F(ST) = 0.060). The highest level of polymorphism was observed in flies from eastern Trat and northern Chiang Mai provinces (69.2%), whereas the lowest level of polymorphism was seen in flies from central Saraburi Province (23.1%). Gene flow between populations varied from 3.27 to 27.53 reproductive migrants per generation. Among the nine populations sampled, no correlation was seen between genetic and geographical distances showing that sampled S. calcitrans fit closely in the same cluster taxa. The electrophoresis of ten isozymes shows a genetic homogeneity of S. calcitrans populations at the scale of Thailand.
采用同工酶淀粉凝胶电泳技术估算了来自泰国的 9 个厩螫蝇(Stomoxys calcitrans)种群(双翅目:蝇科)间的基因流动。在 13 个假定基因座中,检测到 9 个多态性基因座。种群间的遗传分化有限(F(ST) = 0.060)。在东部尖竹汶府和北部清迈府的蝇类中观察到最高水平的多态性(69.2%),而在中部沙拉武里府的蝇类中观察到最低水平的多态性(23.1%)。种群间的基因流动在每一代 3.27 到 27.53 个生殖移民之间变化。在所采样的 9 个种群中,遗传距离和地理距离之间没有相关性,表明所采样的厩螫蝇紧密地聚集在同一分类单元内。十种同工酶的电泳显示,在泰国范围内厩螫蝇种群具有遗传同质性。