Charles University, Prague, Czech Republic.
Neoplasma. 2010;57(5):449-54. doi: 10.4149/neo_2010_05_449.
Primary mediastinal B-cell lymphoma (PMBL) seems to be reliably distinguished from diffuse large B-cell lymphoma (DLBCL) with microarray technology. We measured expression of Fcer2, Pdl2 and Blk genes using real-time quantitative polymerase chain reaction (RTqPCR) on formalin fixed, paraffin embedded material (FFPE) and suggested a formula to discriminate PMBL from DLBCL. For 39/82 included patients the diagnosis of PMBL was expected clinico-pathologically. Diagnosis of 10/39 and 2/43 of clinically considered PMBLs and DLBCLs, respectively, was not genetically confirmed. Compared to confirmed PMBLs, unconfirmed ones showed clinical features similar to DLBCLs, e.g. spleen infiltration (p=0,028) and decreased invasiveness in pericardium (p=0,045). They tended to have more common infradiaphragmatic involvement, less often tumor sclerosis or fluidothorax. There were no immunohistochemical differences between genetically confirmed and unconfirmed PMBLs. New approach of distinguishing PMBL and DLBCL is presented. It is based on expression of three genes in routinely available FFPE material using RTqPCR.
原发性纵隔 B 细胞淋巴瘤 (PMBL) 似乎可以通过微阵列技术可靠地区别于弥漫性大 B 细胞淋巴瘤 (DLBCL)。我们使用实时定量聚合酶链反应 (RTqPCR) 测量了福尔马林固定、石蜡包埋材料 (FFPE) 中 Fcer2、Pdl2 和 Blk 基因的表达，并提出了一种区分 PMBL 和 DLBCL 的公式。对于 39/82 名纳入的患者，预期临床病理诊断为 PMBL。10/39 例临床考虑的 PMBL 和 2/43 例 DLBCL 的诊断未经遗传证实。与确诊的 PMBL 相比，未确诊的 PMBL 具有与 DLBCL 相似的临床特征，例如脾浸润（p=0.028）和心包浸润减少（p=0.045）。它们倾向于更常见的膈下受累，肿瘤硬化或胸腔积液较少。在遗传上确认的和未确认的 PMBL 之间没有免疫组织化学差异。提出了一种新的区分 PMBL 和 DLBCL 的方法。它基于使用 RTqPCR 在常规可用的 FFPE 材料中表达三个基因。
