Harada Akihito, Ohkawa Yasuyuki, Ao Shinpei, Odawara Jun, Okada Seiji, Azuma Masayuki, Nishiyama Yuko, Nakamura Mako, Tachibana Taro
Department of Epigenetics, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hybridoma (Larchmt). 2010 Jun;29(3):255-8. doi: 10.1089/hyb.2009.0117.
Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.
生肌决定因子1（MyoD）是一种具有碱性结构域和螺旋-环-螺旋结构域的生肌调节因子（MRF）。MRF在成肌细胞命运和终末分化中起关键作用。MyoD是一种转录因子，通过与骨骼肌中表达的基因调节因子结合来诱导转录。作为一个主控基因，MyoD还决定骨骼肌的分化。在本研究中，我们采用大鼠髂内侧淋巴结法制备了一种针对MyoD的单克隆抗体。免疫印迹分析表明，我们制备的抗MyoD单克隆抗体能够识别全长MyoD。此外，免疫细胞化学染色显示在骨骼肌分化阶段MyoD的表达发生了变化。这种抗MyoD单克隆抗体有助于进一步研究以阐明MyoD影响骨骼肌分化的机制。
