Kotani Manato, Harada Akihito, Odawara Jun, Azuma Masayuki, Okada Seiji, Nishiyama Yuko, Nakamura Mako, Tachibana Taro, Ohkawa Yasuyuki
Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Hybridoma (Larchmt). 2010 Jun;29(3):259-61. doi: 10.1089/hyb.2009.0107.
Dhx9/NDHII/RHA is a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain. The DEAH protein family plays a critical role in RNA metabolism. DEAH family members function as ATP-dependent RNA helicases and regulation of transcription. In the present study, we report the establishment of a monoclonal antibody specific for Dhx9 using the rat medial iliac lymph node method. Immunoblot analysis using our antibody against Dhx9 detected full-length Dhx9. In addition, immunocytochemical staining using our antibody against Dhx9 revealed the nuclear localization of Dhx9. This monoclonal antibody against Dhx9 will allow for further detailed studies of Dhx9 expression.
Dhx9/NDHII/RHA是DEAH蛋白家族的成员，该家族拥有一个双链RNA结合结构域（dsRBD）和一个解旋酶结构域。DEAH蛋白家族在RNA代谢中起关键作用。DEAH家族成员作为依赖ATP的RNA解旋酶发挥作用，并参与转录调控。在本研究中，我们报告了使用大鼠髂内侧淋巴结法建立针对Dhx9的单克隆抗体。使用我们针对Dhx9的抗体进行免疫印迹分析检测到全长Dhx9。此外，使用我们针对Dhx9的抗体进行免疫细胞化学染色显示Dhx9定位于细胞核。这种针对Dhx9的单克隆抗体将有助于对Dhx9表达进行更详细的研究。
