Khanna-Jain Rashi, Agata Hideki, Vuorinen Annukka, Sándor George K B, Suuronen Riitta, Miettinen Susanna
Regea Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Biokatu 12, 33520, Tampere, Finland.
Growth Factors. 2010 Dec;28(6):437-46. doi: 10.3109/08977194.2010.495719. Epub 2010 Jun 23.
This study was designed to investigate the potential merits of the combined use of bone morphogenetic protein (BMP)-2 or BMP-6 and osteogenic supplements (OS) [dexamethasone, ascorbic acid (AA), and β-glycerophosphate] on osteogenic differentiation of periodontal ligament cells (PDLCs). Osteogenic differentiation was evaluated by quantitative alkaline phosphatase (ALP) assay, alizarin red staining, quantitative calcium assay, and the qRT-PCR analysis for the expression of collagen type I, runt-related transcription factor-2, osteopontin (OPN), and osteocalcin in PDLCs. Culture with BMP-2 or BMP-6+AA increased ALP activity of PDLCs, suggesting their osteo-inductive effects. However, longer duration of culture showed neither of the BMPs induced in vitro mineralization. In contrast, OS were able to increase ALP activity and OPN expressions, and also induced in vitro mineralization. The mineralization ability was not enhanced by the addition of BMP-2 or BMP-6. These findings suggest that the addition of BMP-2 or BMP-6 to OS may not enhance an osteogenic differentiation of hPDLCs.
本研究旨在探讨联合使用骨形态发生蛋白（BMP）-2或BMP-6与成骨补充剂（OS）[地塞米松、抗坏血酸（AA）和β-甘油磷酸]对牙周膜细胞（PDLCs）成骨分化的潜在益处。通过定量碱性磷酸酶（ALP）测定、茜素红染色、定量钙测定以及对PDLCs中I型胶原、 runt相关转录因子-2、骨桥蛋白（OPN）和骨钙素表达的qRT-PCR分析来评估成骨分化。用BMP-2或BMP-6 + AA培养可增加PDLCs的ALP活性，表明它们具有骨诱导作用。然而，较长时间的培养表明，两种BMP均未诱导体外矿化。相比之下，OS能够增加ALP活性和OPN表达，并且还能诱导体外矿化。添加BMP-2或BMP-6并未增强矿化能力。这些发现表明，在OS中添加BMP-2或BMP-6可能不会增强人PDLCs的成骨分化。
