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添加骨形态发生蛋白 2 到抗坏血酸和 β-甘油磷酸盐的补充物并没有增强人脂肪来源干细胞的成骨分化。

Addition of bone morphogenetic protein type 2 to ascorbate and β-glycerophosphate supplementation did not enhance osteogenic differentiation of human adipose-derived stem cells.

机构信息

Department of Dentistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

出版信息

J Appl Oral Sci. 2012 Nov-Dec;20(6):628-35. doi: 10.1590/s1678-77572012000600007.

DOI:10.1590/s1678-77572012000600007
PMID:23329244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3881851/
Abstract

UNLABELLED

Bone morphogenetic protein type 2 (BMP-2) is a potent local factor, which promotes bone formation and has been used as an osteogenic supplement for mesenchymal stem cells.

OBJECTIVES

This study evaluated the effect of a recombinant BMP-2 as well as the endogenous BMP-4 and BMP-7 in the osteogenic differentiation of adipose-derived stem cells (ASCs) in medium supplemented with ascorbate and β-glycerophosphate.

MATERIAL AND METHODS

Human ASCs were treated with osteogenic medium in the presence (ASCs+OM+BMP-2) or absence (ASCs+OM) of BMP-2. The alkaline phosphatase (ALP) activity was determined and the extracellular matrix mineralization was evaluated by Von Kossa staining and calcium quantification. The expressions of BMP-4, BMP-7, Smad1, Smad4, and phosphorylated Smad1/5/8 were analyzed by western blotting. Relative mRNA expressions of Smad1, BMP receptor type II (BMPR-II), osteonectin, and osteocalcin were evaluated by qPCR.

RESULTS

ASCs+OM demonstrated the highest expression of BMP-4 and BMP-7 at days 21 and 7, respectively, the highest levels of BMPR-II mRNA expression at day 28, and the highest levels of Smad1 mRNA at days 14 and 28. ASCs+OM+BMP-2 demonstrated the highest levels of Smad1 mRNA expression at days 1, 7, and 21, the highest expression of Smad1 at day 7, the highest expression of Smad4 at day 14, the highest ALP activity at days 14 and 21, and expression of phosphorylated Smad1/5/8 at day 7. ASCs+OM and ASCs+OM+BMP2 showed similar ALP activity at days 7 and 28, similar osteonectin and osteocalcin mRNA expression at all time periods, and similar calcium depositions at all time periods.

CONCLUSIONS

We concluded that human ASCs expressed endogenous BMP-4 and BMP-7. Moreover, the supplementation of ASCs with BMP-2 did not increase the level of osteogenic markers in the initial (ALP activity), intermediate (osteonectin and osteocalcin), or final (calcium deposition) phases, suggesting that the exogenous addition of BMP-2 did not improve the in vitro osteogenesis process of human ASCs.

摘要

未加说明

骨形态发生蛋白 2（BMP-2）是一种有效的局部因子，可促进骨形成，已被用作间充质干细胞的成骨补充剂。

目的

本研究评估了重组 BMP-2 以及内源性 BMP-4 和 BMP-7 在含抗坏血酸和 β-甘油磷酸盐的培养基中对脂肪来源干细胞（ASCs）成骨分化的影响。

材料和方法

用成骨培养基处理人 ASC（ASCs+OM+BMP-2）或不用 BMP-2（ASCs+OM）。通过碱性磷酸酶（ALP）活性测定和 Von Kossa 染色及钙定量评估细胞外基质矿化。通过 Western blot 分析 BMP-4、BMP-7、Smad1、Smad4 和磷酸化 Smad1/5/8 的表达。通过 qPCR 评估 Smad1、BMP 受体 II（BMPR-II）、骨粘连蛋白和骨钙素的相对 mRNA 表达。

结果

ASCs+OM 在第 21 天和第 7 天分别显示出最高的 BMP-4 和 BMP-7 表达，第 28 天 BMPR-II mRNA 表达水平最高，第 14 天和第 28 天 Smad1 mRNA 表达水平最高。ASCs+OM+BMP-2 在第 1、7 和 21 天显示出最高的 Smad1 mRNA 表达，第 7 天显示出最高的 Smad1 表达，第 14 天显示出最高的 Smad4 表达，第 14 天和第 21 天显示出最高的 ALP 活性，第 7 天显示出磷酸化 Smad1/5/8 的表达。ASCs+OM 和 ASCs+OM+BMP2 在第 7 天和第 28 天显示出相似的 ALP 活性，在所有时间点均显示出相似的骨粘连蛋白和骨钙素 mRNA 表达，在所有时间点均显示出相似的钙沉积。

结论

我们得出结论，人 ASC 表达内源性 BMP-4 和 BMP-7。此外，向 ASC 中补充 BMP-2 并没有增加初始（ALP 活性）、中期（骨粘连蛋白和骨钙素）或最终（钙沉积）阶段的成骨标志物水平，表明外源性添加 BMP-2 并没有改善人 ASC 的体外成骨过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/85ecd5a5b96b/jaos-20-06-0628-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/63c6d6c34e6f/jaos-20-06-0628-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/c5b1f880e523/jaos-20-06-0628-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/926cc5c83628/jaos-20-06-0628-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/85ecd5a5b96b/jaos-20-06-0628-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/63c6d6c34e6f/jaos-20-06-0628-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/c5b1f880e523/jaos-20-06-0628-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/926cc5c83628/jaos-20-06-0628-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c64/3881851/85ecd5a5b96b/jaos-20-06-0628-g04.jpg

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本文引用的文献

1
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2
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J Appl Oral Sci. 2011 May-Jun;19(3):189-94. doi: 10.1590/s1678-77572011000300002.
3
Dental pulp tissue engineering.牙髓组织工程
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Adv Healthc Mater. 2018 Oct;7(19):e1800890. doi: 10.1002/adhm.201800890. Epub 2018 Aug 14.
4
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Int J Mol Sci. 2018 Jul 27;19(8):2200. doi: 10.3390/ijms19082200.
5
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6
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10
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