一种油菜素内酯异构体诱导正常和糖尿病大鼠中己糖激酶 I 的表达。

Induction of hexokinase I expression in normal and diabetic rats by a brassinosteroid isoform.

机构信息

Dept. of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Kalapet, Puducherry 605014, India.

出版信息

Eur J Pharm Sci. 2010 Sep 11;41(1):1-9. doi: 10.1016/j.ejps.2010.05.008. Epub 2010 May 16.

DOI:10.1016/j.ejps.2010.05.008

PMID:20570616

Abstract

This study aimed to investigate the effect of the plant growth regulator 28-homobrassinolide (HB) on the hexokinase I (HK I) enzyme gene expression in the tissues of normal and streptozotocin-induced diabetic rats. Normal and diabetic rats were administered 50 microg of HB for 15 consecutive days. The tissues level of HK I mRNA expression was quantitated by PCR and densitometry analysis, HK I protein expression was quantitated by Western blot and densitometry analysis, localization of HK I was done by immunohistochemistry and HK enzyme activity was determined by coupled enzyme assay. Subchronic treatment of rats with HB enhanced HK I enzyme expression in diabetic rat compared to the control rat. From these experimental evidences, we came to the conclusion that HK I played a vital role in the regulation of blood sugar in streptozotocin-induced diabetic rats. A direct role for hexokinase enzyme activity in the control of diabetes is presented.

摘要

本研究旨在探讨植物生长调节剂 28-高油菜素内酯 (HB) 对正常和链脲佐菌素诱导的糖尿病大鼠组织中己糖激酶 I (HK I) 酶基因表达的影响。正常和糖尿病大鼠连续 15 天给予 50µg HB。通过 PCR 和密度分析定量组织中 HK I mRNA 表达水平，通过 Western blot 和密度分析定量 HK I 蛋白表达水平，通过免疫组织化学定位 HK I，通过偶联酶测定法测定 HK 酶活性。HB 对大鼠的亚慢性治疗增强了糖尿病大鼠中 HK I 酶的表达，与对照组大鼠相比。根据这些实验证据，我们得出结论，HK I 在链脲佐菌素诱导的糖尿病大鼠中血糖调节中发挥着至关重要的作用。己糖激酶酶活性在控制糖尿病方面发挥着直接作用。