28-高油菜素内酯对白血病细胞白三烯合成的治疗作用。
Therapeutic effect of 28-homobrassinolide on leukotriene synthesis in leukemia cells.
作者信息
He Chuan, Liu Zhigang, Huang Jingcao, Liu Ting
机构信息
Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang, Chengdu, 610041, Sichuan, China.
出版信息
Cytotechnology. 2018 Feb;70(1):431-438. doi: 10.1007/s10616-017-0158-4. Epub 2017 Nov 3.
The present study was aimed to investigate the effect of 28-homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C (LTC4) and Leukotriene B4 (LTB4) were determined in the cell and cell lysates. Intracellular Ca was determined in the intact cells. Phospholipases A2 (PLA2) expression was determined in the cells using immunofluorescence. LTC4 and LTB4 were tremendously increased in IgE-loaded 2H3 cells. However, these levels were significantly reduced following treatment with 28-homobrassinolide. The intracellular Ca level was not altered by treatment. Expression of PLA2 was significantly reduced following treatment with 28-homobrassinolide. Taking all these data together, it is suggested that the 28-homobrassinolide may be a potential therapeutic agent to inhibit leukotriene synthesis in 2H3 cells.
本研究旨在探讨28-高油菜素内酯对2H3细胞中白三烯合成的影响。采用磺酰罗丹明B（SRB）法测定细胞活力。在细胞和细胞裂解物中测定白三烯C（LTC4）和白三烯B4（LTB4）。在完整细胞中测定细胞内钙。使用免疫荧光法测定细胞中磷脂酶A2（PLA2）的表达。在负载IgE的2H3细胞中，LTC4和LTB4显著增加。然而，用28-高油菜素内酯处理后，这些水平显著降低。处理后细胞内钙水平未改变。用28-高油菜素内酯处理后，PLA2的表达显著降低。综合所有这些数据，表明28-高油菜素内酯可能是一种潜在的治疗剂，可抑制2H3细胞中的白三烯合成。
Zotero 插件