Indian Institute of Toxicology Research, Lucknow, India.
Toxicol In Vitro. 2010 Sep;24(6):1681-8. doi: 10.1016/j.tiv.2010.05.019. Epub 2010 Jun 4.
Investigations were carried out to examine the suitability of PC12 cells as an in vitro tool to examine 4-hydroxynonenal (4-HNE)-induced toxicity in nervous tissue. On day 8 of differentiation, markers of neural effects and oxidative stress were measured following exposure of PC12 cells to 1-50 microM 4-HNE for 1-8h. Endpoints included dopamine DA-D(2) receptor and glutathione S-transferase (GSTP1-1) protein levels, 4-HNE-protein binding, glutathione (GSH) concentrations and intracellular calcium levels. GSH levels were maximally depleted after 4h. 4-HNE also induced depletion of GSTP1-1 and increased intracellular Ca(++), with the latter seen as early as 1h after exposure. Responses at 8h were not greater than responses at earlier times. The experiments suggest that PC12 cells could be an in vitro tool for understanding toxicant-cell interactions, especially those that result in oxidative stress.
研究旨在探讨 PC12 细胞作为一种体外工具，用于研究神经组织中 4-羟基壬烯醛 (4-HNE) 诱导的毒性的适用性。在分化的第 8 天，将 PC12 细胞暴露于 1-50μM 4-HNE 1-8 小时后，测量神经效应和氧化应激的标志物。终点包括多巴胺 DA-D(2)受体和谷胱甘肽 S-转移酶 (GSTP1-1) 蛋白水平、4-HNE-蛋白质结合、谷胱甘肽 (GSH) 浓度和细胞内钙水平。4 小时后 GSH 水平最大程度地耗竭。4-HNE 还诱导 GSTP1-1 耗竭和细胞内 Ca(++)增加，后者早在暴露后 1 小时就出现。8 小时的反应并不大于早期的反应。实验表明，PC12 细胞可以作为一种体外工具，用于了解毒物-细胞相互作用，特别是那些导致氧化应激的相互作用。
