Frolova Lidia T, Dolmatov Igor Yu
A.V. Zhirmunsky Institute of Marine Biology, FEB RAS, Palchevsky 17, Vladivostok 690041, Russia.
Biol Bull. 2010 Jun;218(3):303-16. doi: 10.1086/BBLv218n3p303.
The morphology and regeneration of the digestive system of the ophiuroid Amphipholis kochii were investigated. The epithelia of the esophagus and stomach of A. kochii were composed of typical enterocytes and mucous cells. The digestive epithelium of the stomach contained two types of granular secretory cells. After autotomy of the disk, the animals retained the esophagus and a small part of the stomach. The dedifferentiation of enterocytes and mucous cells began on the first day after autotomy. On day 3 the cells formed an anlage of stomach around the mouth opening. Later, the stomach anlage grew as a result of cell proliferation. The opening on the aboral side of the body was closed by day 7. By this time differentiating cells were already observed in the stomach lining. The stomach mesothelium was formed by peritoneocytes and myoepithelial cells, which migrated from other coelomic epithelia of the body. Our study showed that the formation of the digestive system in A. kochii during regeneration depended on cells from the esophagus and the stomach remnant. Both enterocytes and mucous cells were able to dedifferentiate, migrate, and proliferate to give rise to the luminal epithelium. The basic mechanism of stomach formation was epithelial morphogenesis.
研究了蛇尾纲动物科氏双鳞蛇尾消化系统的形态学和再生过程。科氏双鳞蛇尾的食管和胃上皮由典型的肠上皮细胞和黏液细胞组成。胃的消化上皮包含两种类型的颗粒分泌细胞。盘部自切后，动物保留了食管和一小部分胃。自切后第一天，肠上皮细胞和黏液细胞开始去分化。第3天，这些细胞在口周围形成胃原基。随后，胃原基因细胞增殖而生长。身体反口侧的开口在第7天关闭。此时，在胃内衬中已观察到分化细胞。胃间皮由腹膜细胞和肌上皮细胞形成，这些细胞从身体的其他体腔上皮迁移而来。我们的研究表明，科氏双鳞蛇尾再生过程中消化系统的形成依赖于食管和胃残余部分的细胞。肠上皮细胞和黏液细胞都能够去分化、迁移和增殖，从而产生管腔上皮。胃形成的基本机制是上皮形态发生。
