干细胞和纤维蛋白浓度对 Medpor 眼眶植入物血管生成的影响。
Effect of stem cells and fibrin concentration on the vascularization of the Medpor orbital implant.
机构信息
Department of Ophthalmology, Inje University, Busan, Republic of Korea.
出版信息
Clin Exp Ophthalmol. 2010 Dec;38(9):885-91. doi: 10.1111/j.1442-9071.2010.02355.x.
BACKGROUND
To determine the effect of adipose-derived adult stem cells (ADASCs) and optimal concentration of fibrin on fibrovascular ingrowth into porous polyethylene orbital implants (Medpor).
METHODS
Medpor sheet treated with O.25% fibrin only and ADASCs in mixtures containing fibrin (0.25%, 0.5% or 1.25%) were applied to a Medpor sheet and implanted in the back of each of 20 athymic nude mice. After 10 days, implants were removed and observed for fibrovascularization and stability. Haemoglobin, collagen and cellular DNA content were determined in quantitative assays.
RESULTS
Haemoglobin, collagen and cellular DNA levels were significantly higher in ADASC group than in the cell-free implant (0.25% fibrin only) group (P < 0.01). The level of haemoglobin and collagen content was significantly higher in the ADASC + 0.5% fibrin group among the ADASC and fibrin mixtures (P < 0.01).
CONCLUSION
ADASCs significantly improved fibrovascularization on Medpor compared with implants alone. Fibrin, used together with ADASCs to potentiate fibrovascularization, was most effective at concentrations of 0.5%.
背景
为了确定脂肪来源的成体干细胞（ADASCs）和最佳浓度的纤维蛋白对多孔聚乙烯眶植入物（Medpor）中纤维血管向内生长的影响。
方法
用 0.25%纤维蛋白处理 Medpor 片，并将 ADASCs 混入含有纤维蛋白（0.25%、0.5%或 1.25%）的混合物中，涂在 Medpor 片上，植入 20 只无胸腺裸鼠的背部。10 天后，取出植入物并观察纤维血管化和稳定性。用定量分析法测定血红蛋白、胶原蛋白和细胞 DNA 含量。
结果
ADASC 组的血红蛋白、胶原蛋白和细胞 DNA 水平明显高于无细胞植入物（仅 0.25%纤维蛋白）组（P<0.01）。在 ADASC 和纤维蛋白混合物中，ADASC+0.5%纤维蛋白组的血红蛋白和胶原蛋白含量水平明显高于其他组（P<0.01）。
结论
与单独植入物相比，ADASCs 显著改善了 Medpor 的纤维血管化。纤维蛋白与 ADASCs 一起使用，可增强纤维血管化作用，在浓度为 0.5%时最有效。
Zotero 插件