State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100094, China.
BMC Plant Biol. 2010 Jun 24;10:123. doi: 10.1186/1471-2229-10-123.
MicroRNAs (miRNAs) are a class of small non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition. MiRNAs can have large-scale regulatory effects on development and stress response in plants.
To test whether miRNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing we cloned the small RNA from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. A total of 153 miRNAs were identified, which belong to 51 known and 81 novel miRNA families. We found that 24 and 12 miRNAs were responsive to powdery mildew infection and heat stress, respectively. We further predicted that 149 target genes were potentially regulated by the novel wheat miRNA.
Our results indicated that diverse set of wheat miRNAs were responsive to powdery mildew infection and heat stress and could function in wheat responses to both biotic and abiotic stresses.
MicroRNAs (miRNAs) 是一类小的非编码调控 RNA，通过引导靶 mRNA 切割或翻译抑制来调节基因表达。miRNAs 可以对植物的发育和应激反应产生大规模的调节作用。
为了测试 miRNAs 是否在调节小麦对白粉病感染和热应激的反应中发挥作用，我们使用 Solexa 高通量测序技术从小麦叶片中克隆了受优势生理菌株 Erysiphe graminis f. sp. tritici (Egt) 感染或热应激处理的小 RNA。共鉴定出 153 个 miRNA，它们属于 51 个已知和 81 个新的 miRNA 家族。我们发现 24 个和 12 个 miRNA 分别对白粉病感染和热应激有反应。我们进一步预测，149 个潜在的靶基因可能受到新的小麦 miRNA 的调控。
我们的结果表明，多种小麦 miRNAs 对白粉病感染和热应激有反应，可以在小麦对生物和非生物胁迫的反应中发挥作用。
