Laboratory of Cell Biology, Ajou University Institute for Medical Sciences, Suwon, Korea.
Department of Biology, Sungshin University, Seoul, Korea.
J Inflamm (Lond). 2010 Jun 24;7:31. doi: 10.1186/1476-9255-7-31.
It has been suggested that the HLA-G molecule is a genetic risk factor for Behcet's disease (BD). In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice. In addition, we investigated siRNA (small interfering RNA) treatment to determine if it inhibited Qa-2 expression, thereby changing the symptoms of mice.
RNA interference (RNAi) and vector transfection were employed to manipulate gene expression in vivo in mice. siRNA (small interfering RNA) or Qa-2 expression vector was applied to inhibit or up-regulate Qa-2 expression, respectively.
The Qa-2 levels in granulocytes were lower in BD-like mice than in normal controls. The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms.
Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice.
有人提出 HLA-G 分子是白塞病 (BD) 的遗传风险因素。在这项研究中，我们评估了 Qa-2 的水平，Qa-2 是一种鼠类非经典 I 类 MHC 分子，可能是 HLA-G 的功能同源物，以确定它是否与类似 BD 的小鼠的各种症状有关。此外，我们研究了 siRNA（小干扰 RNA）治疗，以确定它是否抑制了 Qa-2 的表达，从而改变了小鼠的症状。
采用 RNA 干扰 (RNAi) 和载体转染技术在体内操纵小鼠的基因表达。用 siRNA（小干扰 RNA）或 Qa-2 表达载体分别抑制或上调 Qa-2 的表达。
类似 BD 的小鼠中性粒细胞中的 Qa-2 水平低于正常对照。静脉注射 siRNA（500nmol/只，每 3 天注射 4 次）可特异性沉默 Qa-2，降低 Qa-2 水平并加重类似 BD 的症状。
向小鼠体内注射 siRNA 沉默 Qa-2 可导致类似 BD 的小鼠症状恶化。
