Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, 201203 Shanghai, China.
Phytochemistry. 2010 Aug;71(11-12):1430-7. doi: 10.1016/j.phytochem.2010.05.019. Epub 2010 Jun 21.
A polysaccharide fraction, J6, was isolated from the hot-water extract of flowers of oleander Nerium indicum Mill., using ethanol precipitation, cetyltrimethylammonium bromide (CTAB) complexing, anion-exchange chromatography and gel permeation chromatography. J6 was found to contain L-rhamnose, L-arabinose, D-galactose, and D-galacturonic acid, in the ratio of 10.1:49.8:30.1:10.0. Its structure was investigated by methylation analysis, periodate oxidation, Smith degradation, partial acid hydrolysis, electrospray ionization mass spectrometry and NMR spectroscopic methods. It was found that J6 is an RG-I type polysaccharide, which contains a rhamnogalacturonan backbone, with various branches attached to O-4 of L-rhamnose. The branches probably involve (1-->4)-beta-D-galactan, branched L-arabino-(1-->3)(1-->6)-beta-D-galactan, and (1-->5)-alpha-L-arabinan. J6 stimulated NO production of macrophage RAW264.7 cells in a preliminary test.
从夹竹桃 Nerium indicum Mill. 的热水提取物中，使用乙醇沉淀、十六烷基三甲基溴化铵（CTAB）络合、阴离子交换色谱和凝胶渗透色谱分离得到多糖 J6。J6 含有 L-鼠李糖、L-阿拉伯糖、D-半乳糖和 D-半乳糖醛酸，摩尔比为 10.1:49.8:30.1:10.0。通过甲基化分析、高碘酸盐氧化、Smith 降解、部分酸水解、电喷雾电离质谱和 NMR 光谱方法研究了其结构。发现 J6 是一种 RG-I 型多糖，含有鼠李半乳糖醛酸聚糖主链，各种支链连接到 L-鼠李糖的 O-4 上。这些支链可能涉及（1-->4）-β-D-半乳糖、支链 L-阿拉伯糖（1-->3）（1-->6）-β-D-半乳糖和（1-->5）-α-L-阿拉伯聚糖。在初步试验中，J6 刺激巨噬细胞 RAW264.7 细胞产生 NO。
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