Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 49, Pervomaiskaya Str., 167982 Syktyvkar, Russia.
Carbohydr Polym. 2013 Feb 15;92(2):1817-26. doi: 10.1016/j.carbpol.2012.11.038. Epub 2012 Nov 23.
The pectic polysaccharide named abienan AS-A was isolated from the wood greenery of Abies sibirica using dilute hydrochloric acid (pH 4.0) at 70°C. The structure of abienan AS-A was elucidated using sugar composition analysis, ion-exchange chromatography and partial acid hydrolysis followed by NMR spectroscopy. The linear region of abienan AS-A was shown to contain linear 1,4-α-D-galactopyranosyluronan partially substituted with methyl esters or 3-O-acetyl groups and rhamnogalacturonan blocks consisting of 1,4-α-D-galacturonan partially substituted with methyl ester groups and connected by 2-O-substituted α-rhamnopyranose residues. The branched region of abienan AS-A was found to be made of RG-I. The side chains of RG-I were shown to contain 1,4-β-galactan and branched arabinan. Some 4-O-substituted β-galactopyranose residues were shown to be attached to the 4-position of the 2-O-substituted α-rhamnopyranose residues of the RG-I backbone. The arabinan groups were made up of a 1,5-linked α-L-arabinofuranan backbone that was 3-O-, 2-O-, and 2,3-di-O-substituted with the terminal and 1,3-linked α-L-arabinofuranose residues.
从西伯利亚冷杉的木材绿色部分用稀盐酸（pH 4.0）在 70°C 提取得到名为 abienan AS-A 的果胶多糖。通过糖组成分析、离子交换色谱和部分酸水解以及 NMR 光谱阐明了 abienan AS-A 的结构。abienan AS-A 的线性区域被证明包含线性 1,4-α-D-半乳糖醛酸部分被甲酯或 3-O-乙酰基取代的吡喃糖醛酸聚糖和由 1,4-α-D-半乳糖醛酸组成的鼠李半乳糖醛酸聚糖块部分被甲酯取代并通过 2-O-取代的α-鼠李吡喃糖残基连接。abienan AS-A 的支化区域被发现是 RG-I。RG-I 的侧链含有 1,4-β-半乳糖和支链阿拉伯聚糖。一些 4-O-取代的β-半乳糖吡喃糖残基被认为连接到 RG-I 主链 2-O-取代的α-鼠李吡喃糖残基的 4 位。阿拉伯聚糖由 1,5-连接的α-L-阿拉伯呋喃聚糖主链组成，该主链被末端和 1,3-连接的α-L-阿拉伯呋喃糖残基 3-O-、2-O-和 2,3-二取代。
