Farshad Shohreh, Rasouli Manoochehr, Jamshidzadeh Akram, Hosseinkhani Ayda, Japoni Aziz, Alborzi Abdolvahab, Taghavi Alireza, Kazemi Asl Hossein, Ranjbar Reza
Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Immunol. 2010 Jun;7(2):96-108.
Previous studies imply that IL-1 and IL-8 gene variations may play a crucial role in the genetic predisposition to different gastric disorders upon H. pylori infection.
The aim of this study was to determine the potential association between the prevalence of certain polymorphic sites and the risk of gastric disorders in Iranian population.
One hundred and forty three unrelated individuals with different gastric disorders and 374 normal individuals with no gastric disorders and with a negative serology test for H. pylori (control group) were studied for the association between IL-1ß (+3953 C/T) and IL-8 (-251 A/T) gene polymorphisms and H. pylori-mediated gastritis and gastric ulcer. An analysis of genotype frequency for these genes was performed using RFLP-PCR.
Based on the data obtained from culture and pathologic findings, the patients were classified into three subpopulations: H. pylori(+) non-ulcerative gastritis(+), H. pylori(+) ulcerative gastritis(+) and H. pylori(-) non-ulcerative gastritis(+). A significantly higher frequency of TT genotype (p=0.02) in IL-1ß +3953 in H. pylori(+) ulcerative gastritis(+) was revealed compared to the control group. There were no significant differences among other subpopulations. No significant differences in allele and genotype frequencies of IL-8 (-251A/T) were found among the patients.
The data suggest that TT genotype in IL-1ß +3953 may be a major contributing genetic risk factor for H. pylori induced gastric ulcer. Moreover, the role of other bacterial and host response factors, such as bacterial adherence peptides, host chemokines, and genes involved in gastric acid secretion, must be further investigated in different ethnic populations.
先前的研究表明，白细胞介素-1（IL-1）和白细胞介素-8（IL-8）基因变异可能在幽门螺杆菌感染后不同胃部疾病的遗传易感性中起关键作用。
本研究旨在确定伊朗人群中某些多态性位点的患病率与胃部疾病风险之间的潜在关联。
对143名患有不同胃部疾病的无亲缘关系个体和374名无胃部疾病且幽门螺杆菌血清学检测呈阴性的正常个体（对照组）进行研究，以探讨白细胞介素-1β（+3953 C/T）和白细胞介素-8（-251 A/T）基因多态性与幽门螺杆菌介导的胃炎和胃溃疡之间的关联。使用限制性片段长度多态性聚合酶链反应（RFLP-PCR）对这些基因的基因型频率进行分析。
根据培养和病理检查结果，患者被分为三个亚组：幽门螺杆菌（+）非溃疡性胃炎（+）、幽门螺杆菌（+）溃疡性胃炎（+）和幽门螺杆菌（-）非溃疡性胃炎（+）。与对照组相比，幽门螺杆菌（+）溃疡性胃炎（+）患者中白细胞介素-1β +3953位点的TT基因型频率显著更高（p = 0.02）。其他亚组之间无显著差异。患者中白细胞介素-8（-251A/T）的等位基因和基因型频率无显著差异。
数据表明，白细胞介素-1β +3953位点的TT基因型可能是幽门螺杆菌诱导胃溃疡的主要遗传风险因素。此外，其他细菌和宿主反应因素，如细菌黏附肽、宿主趋化因子以及参与胃酸分泌的基因，在不同种族人群中的作用仍需进一步研究。
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