Ramis Ivy Bastos, Vianna Júlia Silveira, Halicki Priscila Cristina Bartolomeu, Lara Caroline, Tadiotto Thássia Fernanda, da Silva Maciel João Batista, Gonçalves Carla Vitola, von Groll Andrea, Dellagostin Odir Antônio, da Silva Pedro Eduardo Almeida
Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil.
J Infect Dev Ctries. 2015 Sep 29;9(10):1108-16. doi: 10.3855/jidc.6123.
Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases.
Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR.
It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B. No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found.
We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.
幽门螺杆菌感染与胃炎、消化性溃疡病及胃癌相关。损伤的严重程度由环境/行为因素、细菌致病基因和宿主基因多态性之间的相互作用决定，这些因素会影响炎性细胞因子的分泌水平。因此，本研究旨在鉴定白细胞介素-1β（IL-1B）基因和白细胞介素-1受体拮抗剂（IL-1RN）基因的多态性及其与幽门螺杆菌感染、幽门螺杆菌cagA基因和胃十二指肠疾病的关联。
对151例幽门螺杆菌感染患者和76例未感染个体的胃活检样本进行分析。通过组织学和聚合酶链反应（PCR）诊断幽门螺杆菌感染。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测IL-1B基因-511、-31和+3954位点的多态性，并通过PCR对IL-1RN基因的可变数目串联重复序列（VNTR）多态性进行分析。
观察到-511位点的T/T基因型和-31位点的C/C基因型与幽门螺杆菌感染以及幽门螺杆菌阳性患者胃炎风险增加相关。此外，携带cagA基因的幽门螺杆菌阳性患者的菌株与IL-1B基因-511位点的T/T基因型显著相关。未发现IL-1B基因+3954位点和IL-1RN基因的多态性与幽门螺杆菌感染及严重胃部疾病风险之间存在关联。
我们证明，IL-1B基因启动子区域（-511和-31位点）的多态性与幽门螺杆菌感染风险增加以及幽门螺杆菌阳性患者的胃炎相关。
