白细胞介素 1B-511C/-31T 单倍型与幽门螺杆菌 vacA 基因型与胃溃疡和慢性胃炎的关系。
Association of IL1B -511C/-31T haplotype and Helicobacter pylori vacA genotypes with gastric ulcer and chronic gastritis.
机构信息
Laboratorio de Investigación Clínica, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av, Lázaro Cárdenas S/N, Ciudad Universitaria, Colonia Haciendita, Chilpancingo, Guerrero, C,P, 39090, México.
出版信息
BMC Gastroenterol. 2010 Oct 27;10:126. doi: 10.1186/1471-230X-10-126.
BACKGROUND
The association between proinflammatory cytokine gene polymorphisms and gastric diseases related to Helicobacter pylori varies by population and geographic area.Our objective was to determine if the IL-1B -511 T>C and -31 C>T polymorphisms and H. pylori vacA genotypes are associated with risk of chronic gastritis and gastric ulcer in a Mexican population.
METHODS
We conducted endoscopic studies in 128 patients with symptoms of dyspepsia. We took two biopsies from the body, antrum, or ulcer edge from each patient, and classified our histopathological findings according to the Sydney System. H. pylori infection and vacA genotyping were accomplished via PCR from total DNA of the gastric biopsies. We confirmed the presence of anti-H. pylori serum IgG and IgM in 102 control subjects. In both case subjects and control subjects, the IL-1B -511 T>C polymorphism was genotyped by PCR-RFLPs and the IL-1B -31 C>T polymorphism was genotyped by pyrosequencing.
RESULTS
Sixty-two point seven (62.7%) of the 102 control subjects were H. pylori-seropositive. Among the case subjects, 100 were diagnosed with chronic gastritis and 28 with gastric ulcer. We found that 77% of the patients with chronic gastritis and 85.7% of the patients with gastric ulcer were H. pylori-positive. The predominant H. pylori genotype was vacA s1m1 (58.4%) and the most frequent subtype was vacA s1. The -511 TC, (rs16944 -511 T>C) genotype and the -511C allele were associated with chronic gastritis (OR = 3.1, 95% CI = 1.4-6.8 and OR = 3.0, 95% CI = 1.4-6.0, respectively). The subjects carrying -31T (rs1143627 -31 C>T) were found to be at a higher risk of having chronic gastritis (OR = 2.8, 95% CI = 1.3-5.8). The IL-1B -511C/-31T haplotype was associated with chronic gastritis (OR = 2.1, 95% CI = 1.2-3.8) but not with gastric ulcer.
CONCLUSIONS
The H. pylori vacA genotypes identified herein were similar to those reported for other regions of Mexico. The vacA s1m1 genotype was not associated with gastric ulcer. In the southern Mexican population, the IL-1B -511C and -31T alleles and the -511C/-31T and -511T/-31T haplotypes are associated with increased risk of chronic gastritis and gastric ulcer.
背景
与幽门螺杆菌相关的胃疾病与促炎细胞因子基因多态性的关联因人群和地理区域而异。我们的目的是确定白细胞介素-1B（IL-1B）-511T>C 和-31C>T 多态性和 H. pylori vacA 基因型是否与墨西哥人群中慢性胃炎和胃溃疡的风险相关。
方法
我们对 128 例有消化不良症状的患者进行了内镜研究。我们从每位患者的体部、胃窦或溃疡边缘取了两个活检，并根据悉尼系统对我们的组织病理学发现进行了分类。通过从胃活检的总 DNA 进行 PCR，我们完成了 H. pylori 感染和 vacA 基因分型。我们在 102 名对照受试者中确认了存在抗 H. pylori 血清 IgG 和 IgM。在病例组和对照组中，IL-1B-511T>C 多态性通过 PCR-RFLP 进行基因分型，IL-1B-31C>T 多态性通过焦磷酸测序进行基因分型。
结果
在 102 名对照受试者中，有 62.7%为 H. pylori 血清阳性。在病例组中，有 100 例被诊断为慢性胃炎，28 例为胃溃疡。我们发现，77%的慢性胃炎患者和 85.7%的胃溃疡患者为 H. pylori 阳性。主要的 H. pylori 基因型为 vacA s1m1（58.4%），最常见的亚型为 vacA s1。-511TC（rs16944-511T>C）基因型和-511C 等位基因与慢性胃炎相关（OR=3.1，95%CI=1.4-6.8 和 OR=3.0，95%CI=1.4-6.0）。携带-31T（rs1143627-31C>T）的受试者被发现患慢性胃炎的风险更高（OR=2.8，95%CI=1.3-5.8）。IL-1B-511C/-31T 单倍型与慢性胃炎相关（OR=2.1，95%CI=1.2-3.8），但与胃溃疡无关。
结论
本文确定的 H. pylori vacA 基因型与墨西哥其他地区报道的相似。vacA s1m1 基因型与胃溃疡无关。在墨西哥南部人群中，IL-1B-511C 和-31T 等位基因以及-511C/-31T 和-511T/-31T 单倍型与慢性胃炎和胃溃疡的风险增加相关。
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