College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
J Environ Sci Health B. 2010 Aug;45(6):508-15. doi: 10.1080/03601234.2010.493476.
A rapid and sensitive indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method based on quantum dots as the fluorescence label coupled with secondary antibody (Ab(2)) for the detection of chlorpyrifos in drinking water has been developed. The cFLISA method allowed for chlorpyrifos determination in a liner working range of 15.2-205.5 ng mL(-1). The 50 % inhibition value (IC(50)) and the limit of detection (LOD) of the cFLISA were 50.2 ng mL(-1) and 8.4 ng mL(-1), while the IC(50) and the LOD of the conventional enzyme linked immunosorbent assay (ELISA) were 95.3 ng- mL(-1) and 16.2 ng mL(-1), respectively. When the concentrations of chlorpyrifos were 200, 100 and 50 ng mL(-1), the recoveries ranged from 90.8 % to 108.2 % with a coefficient of variation (CV) of 7.5 %-15.2 %. In water sample analysis, the results of cFLISA were similar to those obtained from a cELISA and a high performance liquid chromatography (HPLC) method, while the detection time by cFLISA was reduced 0.5 h compared with ELISA. It showed that cFLISA could be used as a new screening method for the detection of pesticide residue.
基于量子点作为荧光标记物并结合二级抗体（Ab(2)）的快速灵敏的间接竞争荧光酶联免疫吸附测定法（cFLISA）已被开发用于检测饮用水中的毒死蜱。cFLISA 方法允许在 15.2-205.5ngmL(-1)的线性工作范围内测定毒死蜱。cFLISA 的 50%抑制值（IC(50)）和检测限（LOD）分别为 50.2ngmL(-1)和 8.4ngmL(-1)，而常规酶联免疫吸附测定（ELISA）的 IC(50)和 LOD 分别为 95.3ng- mL(-1)和 16.2ngmL(-1)。当毒死蜱浓度为 200、100 和 50ngmL(-1)时，回收率在 90.8%-108.2%之间，变异系数（CV）为 7.5%-15.2%。在水样分析中，cFLISA 的结果与 cELISA 和高效液相色谱（HPLC）方法的结果相似，而与 ELISA 相比，cFLISA 的检测时间缩短了 0.5 小时。结果表明，cFLISA 可作为检测农药残留的一种新的筛选方法。
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