Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, PR China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, PR China.
Food Chem. 2014 Mar 1;146:314-9. doi: 10.1016/j.foodchem.2013.09.048. Epub 2013 Sep 19.
A fluoroimmunoassay towards aflatoxin B1 (AFB1) was presented using quantum dots as the fluorescent label. The CdTe QDs were successfully linked to the monoclonal antibody against AFB1. Based on the conjugated complexes, a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) was developed for AFB1 detection. The 50% inhibition value (IC50) of the cFLISA was 0.149ng/mL in peanuts matrix. The method performance included the limit of detection (LOD) of 0.016ng/mL and considerable recoveries of 85-117% at three fortification levels (0.075, 0.15, and 0.3ng/g) from spiked AFB1 blank peanuts samples, along with coefficients of variation (CVs) below 10%. The cFLISA provided an alternative of rapid and sensitive detection for AFB1 and, moreover provided great potential for multiplexed mycotoxins determination simultaneously.
建立了一种基于量子点荧光标记的黄曲霉毒素 B1（AFB1）荧光免疫分析方法。CdTe量子点成功地与针对 AFB1 的单克隆抗体偶联。基于偶联复合物，建立了一种用于检测 AFB1 的新型直接竞争荧光酶联免疫吸附测定法（cFLISA）。在花生基质中，cFLISA 的 50%抑制值（IC50）为 0.149ng/mL。该方法的性能包括检测限（LOD）为 0.016ng/mL，在添加 AFB1 空白花生样品的三个加标水平（0.075、0.15 和 0.3ng/g）下，回收率为 85-117%，变异系数（CV）低于 10%。cFLISA 为 AFB1 的快速灵敏检测提供了一种替代方法，而且为同时测定多种真菌毒素提供了很大的潜力。
