Yao Jingjing, Xing Guangxu, Han Junling, Sun Yaning, Wang Fangyu, Deng Ruiguang, Hu Xiaofei, Zhang Gaiping
Henan Academy of Agriculture Science/Key laboratory of animal Immunology, Ministry of Agriculture/Henan key laboratory of animal Immunology, Zhengzhou, 450002, China.
The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453100, China.
J Biophotonics. 2017 May;10(5):657-663. doi: 10.1002/jbio.201600005. Epub 2016 May 31.
Novel direct and indirect competitive fluorescence-linked immunosorbent assays (cFLISA and icFLISA) for detection of ochratoxin A (OTA) were described using CdTe quantum dots (QDs) as fluorescent label. CdTe QDs were successfully synthesized, which had an emission wavelength of 615 nm. The high purity monoclonal antibody against OTA was prepared through cell thawing and the octylic acid-ammonium sulfate method. The OTA MAbs were successfully coupled with CdTe QDs, and which also retained the original biological activity. The 50% inhibition values (IC ) of the cFLISA and icFLISA were 0.630 ng/mL, 0.234 ng/mL, the limits of detection (LOD) were 7.06 × 10 and 4.15 × 10 ng/mL, and detection ranges were 7.06 × 10 - 18.34 ng/mL and 4.15 × 10 - 4.88 ng/mL, in-order. The recoveries were 96.0-104.7% along with coefficients of variation (CVs) below 10%. The FLISA provided novel method for determination of OTA and the potential of MAb-CdTe QDs for the establishment of fluorescent immunochromatographic test strip.
描述了使用碲化镉量子点（QDs）作为荧光标记物检测赭曲霉毒素A（OTA）的新型直接和间接竞争荧光酶联免疫吸附测定法（cFLISA和icFLISA）。成功合成了发射波长为615nm的碲化镉量子点。通过细胞解冻和辛酸 - 硫酸铵法制备了高纯度的抗OTA单克隆抗体。OTA单克隆抗体成功与碲化镉量子点偶联，并且还保留了原始生物活性。cFLISA和icFLISA的50%抑制值（IC）分别为0.630ng/mL、0.234ng/mL，检测限（LOD）分别为7.06×10和4.15×10 ng/mL，检测范围依次为7.06×10 - 18.34ng/mL和4.15×10 - 4.88ng/mL。回收率为96.0 - 104.7%，变异系数（CV）低于10%。该荧光酶联免疫吸附测定法为OTA的测定提供了新方法，以及单克隆抗体 - 碲化镉量子点用于建立荧光免疫层析试纸条的潜力。
