Song Xiaomei, Li Lin, Yang Guangming, Cai Baochang
Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2010 Apr;35(7):885-7. doi: 10.4268/cjcmm20100718.
To determinate the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas. The HPLC separation was performed on a Inertsil ODS-sp column (4.6 mm x 150 mm, 5 microm). A mixture of acetonitrile and 0.2% phosphoric acid solution (35:65) as the mobile phase the column. Temperature was set in 30 degrees C. The flow rate was 1.0 mL x min(-1), and the wave length of the detector is 203 nm. The content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from Meixian, Shaanxi is the highest and the lowest is from Enshi, Hubei. There have most differerence among the content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas.
测定不同产地大叶三七中竹节人参皂苷IVa的含量。采用Inertsil ODS-sp柱（4.6 mm×150 mm，5μm）进行高效液相色谱分离。以乙腈和0.2%磷酸溶液（35:65）的混合液作为流动相。柱温设定为30℃。流速为1.0 mL·min⁻¹，检测器波长为203 nm。陕西眉县大叶三七中竹节人参皂苷IVa的含量最高，湖北恩施的含量最低。不同产地大叶三七中竹节人参皂苷IVa的含量差异最大。
