Hu Yuanlang, Yuan Ding, He Yumin, Wang Hongwu, Sun Zhiwei, Zhang Changcheng
Medical College of China Three Gorges University, Yichang 443002, China.
Zhongguo Zhong Yao Za Zhi. 2010 Apr;35(8):1009-13. doi: 10.4268/cjcmm20100815.
The aim was to establish the chromatographic fingerprint of Rhizoma Panacis Japonici for its quality evaluation. Analysis was performed on a YMC-Pack ODS-AQ column (4.6 mm x 250 mm, 5 microm) eluted with the mobile phases of acetonitrile(A) and 5% acetic acid solution (B) at in gradient a flow rate of 1.0 mL x min(-1). The elution program was as follow: 0-5 min, changed from 20% A to 40% A, 5-20 min, kept 40% A. The temperature of column was 30 degrees C. The temperate of drift tube was 40 degrees C, and the nitrogen pressure was 33 Psi. Ten batches of Rhizoma Panacis japonici were determined. The HPLC-ELSD chromatographic fingerprint of chemical constituents was established from the 10 batches of Rhizoma Panacis japonici and it had 9 characteristic common peaks. The 10 batches of samples were classified into 2 cluster by cluster analysis. Furthermore, five known chemical constuituents were identified after isolation and purification by means of silica gel column chromatography, and semi-preparative high performance liquid chromatography. They were panaxsaponins Re, chikusetsusaponins IV, IVa, V and pjs-2, respectively. This study provided experimental data for comprehensive evaluation of the quality of Rhizoma Panacis japonici.
目的是建立竹节参的色谱指纹图谱以用于其质量评价。采用YMC-Pack ODS-AQ柱（4.6 mm×250 mm，5μm）进行分析，以乙腈（A）和5%乙酸溶液（B）为流动相，梯度洗脱，流速为1.0 mL·min⁻¹。洗脱程序如下：0 - 5分钟，A由20%变为40%；5 - 20分钟，保持40%的A。柱温为30℃。漂移管温度为40℃，氮气压力为33 Psi。测定了10批竹节参。建立了10批竹节参的HPLC-ELSD化学成分色谱指纹图谱，共有9个特征共有峰。通过聚类分析将10批样品分为2类。此外，通过硅胶柱色谱和半制备高效液相色谱分离纯化后，鉴定了5种已知化学成分。它们分别是人参皂苷Re、竹节人参皂苷IV、IVa、V和pjs-2。本研究为竹节参质量的综合评价提供了实验数据。
