Liu Ping, He Xinrong, Guo Mei
Traditional Chinese Medicine Pharmacy of PLA General Hospital, Beijing 100853, China.
Zhongguo Zhong Yao Za Zhi. 2010 Apr;35(7):904-7. doi: 10.4268/cjcmm20100723.
To investigate the correlation effects between single or combined administration of Calculus Bovis or zolpidem and changes of inhibitive neurotransmitter in rat striatum corpora.
Sampling from rat striatum corpora was carried out through microdialysis. The content of two inhibitive neurotransmitters in rat corpus striatum- glycine (Gly) and gama aminobutyric acid (GABA), was determined by HPLC, which involved pre-column derivation with orthophthaladehyde, reversed-phase gradient elution and fluorescence detection.
GABA content of rat striatum corpora in Calculus Bovis group was significantly increased compared with saline group (P < 0.01). GABA content of zolpidem group and Calculus Boris plus zolpidem group were increased largely compared with saline group as well (P < 0.05). GABA content of Calculus Bovis group was higher than combination group (P < 0.05). GABA content of zolpidem group was not significantly different from combination group. Gly content of Calculus Bovis or zolpidem group was markedly increased compared with saline group or combination group (P < 0.05).
Contents of two inhibitive neurotransmitters in rat striatum corpora were all significantly increased in Calculus Bovis group, zolpidem group and combination group. The magnitude of increase was lower in combination group than in Calculus Bovis group and Zolpidem group, suggesting that Calculus Bovis promoted encephalon inhibition is more powerful than zolpidem. The increase in two inhibitive neurotransmitters did not show reinforcing effect in combination group, suggesting that Calculus Bovis and zolpidem may compete the same receptors. Therefore, combination of Calculus Bovis containing drugs and zolpidem has no clinical significance. Calculus Bovis shouldn't as an aperture-opening drugs be used for resuscitation therapy.
探讨牛黄单味或与唑吡坦联合给药对大鼠纹状体中抑制性神经递质变化的相关影响。
采用微透析法从大鼠纹状体取样。采用高效液相色谱法测定大鼠纹状体中两种抑制性神经递质——甘氨酸（Gly）和γ-氨基丁酸（GABA）的含量，该方法包括邻苯二甲醛柱前衍生、反相梯度洗脱和荧光检测。
牛黄组大鼠纹状体中GABA含量与生理盐水组相比显著升高（P<0.01）。唑吡坦组和牛黄加唑吡坦组的GABA含量与生理盐水组相比也有大幅升高（P<0.05）。牛黄组的GABA含量高于联合用药组（P<0.05）。唑吡坦组的GABA含量与联合用药组无显著差异。牛黄组或唑吡坦组的Gly含量与生理盐水组或联合用药组相比显著升高（P<0.05）。
牛黄组、唑吡坦组和联合用药组大鼠纹状体中两种抑制性神经递质的含量均显著升高。联合用药组的升高幅度低于牛黄组和唑吡坦组，提示牛黄促进脑抑制的作用比唑吡坦更强。联合用药组中两种抑制性神经递质的升高未表现出增强作用，提示牛黄和唑吡坦可能竞争相同的受体。因此，含牛黄的药物与唑吡坦联合使用无临床意义。牛黄不应作为开窍药用于苏醒治疗。
