Zhang Na, Liu Ping, He Xinrong
Traditional Chinese Medicine Pharmacy, Drugs Supply Centre of the PLA Hospital, Beijing 100853, China.
Zhongguo Zhong Yao Za Zhi. 2011 Nov;36(22):3180-3.
To research the content changes of excitatory neurotransmitter and inhibitory neurotransmitter in corpus striatum of rats after single-used borneol and combining it with diazepam in hope of comprehending the activity of borneol on central nervous system and to observe whether borneol could increase the penetration of other drugs into the brain.
The content of four amino acids neurotransmitters in corpus striatum of rats were sampled by brain microdialysis technology at different time after administration and were determined by RP-HPLC which involved pre-column derivation with orthophthaladehyde (OPA), using phosphate gradient elution and fluorescence detection to detect the content of excitatory neurotransmitter aspartate (Asp), glutamate (Glu) and inhibitory neurotransmitter glycine (Gly), gamma-aminobutyric acid (GABA) in standards and samples and carry on statistical analysis.
The content of both Gly and GABA in corpus striatum of rats with borneol increased significantly, compared with diazepam group (P < 0.05), while Asp and Glu showed no significant difference.
Borneol can improve permeability of diazepam through BBB.
研究大鼠单次使用冰片及冰片与地西泮合用后纹状体内兴奋性神经递质和抑制性神经递质的含量变化，以了解冰片对中枢神经系统的作用活性，并观察冰片是否能增加其他药物的脑内渗透率。
采用脑微透析技术，于给药后不同时间点采集大鼠纹状体内4种氨基酸类神经递质，用邻苯二甲醛（OPA）柱前衍生、磷酸盐梯度洗脱、荧光检测的反相高效液相色谱（RP-HPLC）法测定标准品及样品中兴奋性神经递质天冬氨酸（Asp）、谷氨酸（Glu）和抑制性神经递质甘氨酸（Gly）、γ-氨基丁酸（GABA）的含量，并进行统计分析。
冰片组大鼠纹状体内Gly和GABA含量均显著升高，与地西泮组比较差异有统计学意义（P<0.05），而Asp和Glu含量差异无统计学意义。
冰片可提高地西泮通过血脑屏障的通透性。
