Wang Lan, Liu Li-Sha, Wu Di, An Fang-yu
Gansu College of TCM, Lanzhou 730000, China.
Zhong Yao Cai. 2010 Feb;33(2):171-3.
Study on chromosome number, karyotype of Gentiana straminea for the first time. Compare karyotypes of Gentiana straminea, Gentiana macrophylla and Gentiana dahurica. Provide cytological evidence for further studies on genetics and evolution.
Soak the root tip in 0.002 mol/L 8-oxychinolin solution for 5.7 h. Decomposed in 2.5% mixed enzyme solution for 1.6 h at 25 degrees C and use Hypotonic treatment for 3 h in refrigerator. At last, make specimen slides by the Air-drying technique. Sections combined with micrograph were used to analyze chromosome.
The karyotypes formula of Gentiana straminea is K(2n) = 26 = 2M + 24 m, the AS. K was 52.68%, which belong to "1A" type.
Compare karyotypes of Gentiana straminea, Gentiana macrophylla and Gentiana dahurica, the result showed that Gentiana macrophylla lives in highest stage of evolution. Gentiana straminea is intermediate between Gentiana macrophylla and Gentiana dahurica.
首次对麻花艽染色体数目、核型进行研究。比较麻花艽、秦艽和达乌里秦艽的核型。为进一步开展遗传学及进化研究提供细胞学依据。
将根尖置于0.002 mol/L 8-羟基喹啉溶液中浸泡5.7 h。于25℃在2.5%混合酶液中解离1.6 h，并在冰箱中进行3 h的低渗处理。最后采用空气干燥法制作标本玻片。结合切片与显微照片分析染色体。
麻花艽核型公式为K(2n)=26 = 2M + 24m，不对称系数AS.K为52.68%，属于“1A”型。
比较麻花艽、秦艽和达乌里秦艽的核型，结果表明秦艽处于进化的最高阶段。麻花艽介于秦艽和达乌里秦艽之间。
