Ji Keping, Liu Lisha, Zhang Xiling, Wang Lan
Gansu College of Traditional Chinese Medicine, Lanzhou 730000.
Zhong Yao Cai. 2003 Jan;26(1):11-4.
To analyse the electrophoresis atlas of the PCR amplified products of the internal transcribed spacerl regions of the rRNA gene in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea in Gansu.
With synthetic peculiar PCR primer, internal transcribed spacerl sequences of rRNA gene were amplified in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea. And then the electrophoretic atlas of nPCR amplified products on the agar sugar gel were analysed.
The electrophoresis atlas showed that the lengths of the internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea had the same region 360 bp. The length had enough genetic information to analyse base sequence.
The PCR amplified products electrophoretic atlas of internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea can be used as one of signs for identification on molecule level.
分析甘肃家种和野生秦艽、麻花秦艽核糖体RNA基因内转录间隔区1（ITS1）的DNA经PCR扩增产物的电泳图谱。
用合成的特异PCR引物，对家种和野生秦艽、麻花秦艽的DNA进行rRNA基因内转录间隔区1序列扩增，然后分析琼脂糖凝胶上PCR扩增产物的电泳图谱。
电泳图谱显示，秦艽、麻花秦艽DNA中rRNA基因内转录间隔区1的长度有相同区域360bp，该长度有足够的遗传信息用于分析碱基序列。
秦艽、麻花秦艽DNA中rRNA基因内转录间隔区1的PCR扩增产物电泳图谱可作为分子水平鉴定的标志之一。
