Namiduru Emine Siber, Namiduru Mustafa, Tarakçioğlu Mehmet, Toy Armağan
University of Gaziantep, Faculty of Medicine, Department of Biochemistry, Gaziantep, Turkey.
Clin Lab. 2010;56(5-6):207-13.
The aim of the present study was to determine erythrocyte glutathione, superoxide dismutase, catalase, glutathione peroxidase, and serum total antioxidant response levels in a large chronic viral hepatitis group who had no antiviral treatment, and also the relationship of these parameters with viral load, fibrosis score, and necro-inflammation of the liver.
200 patients who were diagnosed with chronic viral hepatitis and 107 healthy subjects were included in this study. Antioxidant parameters were measured spectrophotometrically. The viral load was assayed using a polymerase chain reaction technique. Histopathologic findings in the liver were scored as necro-inflammatory activity and fibrosis according to Ishak-Knodell score.
Erythrocyte superoxide dismutase, catalase, glutathione peroxidase activities, glutathione, and serum total antioxidant response levels were significantly lower in patients than in controls (p < 0,001). Additionally, no significant correlation was found between these markers and viral load, necro-inflammation, and fibrosis of the liver in patients with chronic viral hepatitis.
Our data suggest the insufficiency of an antioxidant barrier in patients with chronic viral hepatitis, but the decrease in antioxidant systems was not correlated with viral load, necro-inflammatory activity, and fibrosis score in the liver.
本研究旨在测定一大组未接受抗病毒治疗的慢性病毒性肝炎患者的红细胞谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶以及血清总抗氧化反应水平，同时确定这些参数与病毒载量、纤维化评分及肝脏坏死性炎症之间的关系。
本研究纳入了200例被诊断为慢性病毒性肝炎的患者及107名健康受试者。采用分光光度法测定抗氧化参数。使用聚合酶链反应技术检测病毒载量。根据Ishak-Knodell评分将肝脏组织病理学检查结果分为坏死性炎症活动度和纤维化程度。
患者的红细胞超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性、谷胱甘肽以及血清总抗氧化反应水平显著低于对照组（p < 0.001）。此外，在慢性病毒性肝炎患者中，这些标志物与病毒载量、肝脏坏死性炎症及纤维化之间未发现显著相关性。
我们的数据表明慢性病毒性肝炎患者存在抗氧化屏障不足的情况，但抗氧化系统的降低与病毒载量、坏死性炎症活动度及肝脏纤维化评分无关。
