Department of Genome Sciences, University of Washington, Seattle, Washington 98195-8050, USA.
Anal Chem. 2010 Jul 15;82(14):6281-6. doi: 10.1021/ac100461c.
A novel excite-coupled Trapping Ring Electrode Cell (eTREC) was designed and developed. eTREC technology provides greater linearity in the excitation electric field along with minimized variation in radial trapping field during detection. The variation in the radial trapping electric field is reduced through postexcitation modulation of the trapping potentials applied to the Trapping Ring Electrode Cell (TREC). Linearization of the electric field generated during radio frequency (RF) excitation is accomplished by coupling the RF excitation to a novel electrode arrangement superimposed onto the trapping rings of a TREC. The coupling of RF excitation to the trap plates effectively reduces z-axis ejection and allows for a more uniform postexcitation radius for the entire ion population. Using this technology, sensitivity was increased by >50%, resolution of (13)C(2) and (34)S fine structure peaks was achieved with the peptide MMMMG (approximately 330,000 RP) on a 3 T system, and the limit of detection was significantly reduced.
设计并开发了一种新型的激发偶联俘获环电极细胞（eTREC）。eTREC 技术在检测过程中提供了更大的激发电场线性度，同时最小化了径向俘获场的变化。通过对施加到俘获环电极细胞（TREC）的俘获电位进行后激发调制，减少了径向俘获电场的变化。通过将射频（RF）激发耦合到叠加在 TREC 俘获环上的新型电极布置，实现了在 RF 激发过程中产生的电场的线性化。将 RF 激发耦合到陷阱板有效地减少了 z 轴喷射，并允许整个离子种群具有更均匀的后激发半径。使用这项技术，灵敏度提高了超过 50%，在 3T 系统上，对肽 MMMMG（约 330,000 RP）实现了（13）C（2）和（34）S 精细结构峰的分辨率，并显著降低了检测限。
