Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
Langmuir. 2010 Jul 20;26(14):11609-14. doi: 10.1021/la1012582.
We present a novel bonding technique for poly(dimethylsiloxane) (PDMS)-based devices employing chemical surface modifications at room temperature. PDMS surfaces were functionalized to present primary amine groups, and glass or gold substrates were functionalized to present carboxylic acid groups. Irreversible bonding was achieved by bringing the two surfaces in contact and reacting at room temperature to form peptide bonds between the substrates. Shear tests reveal the bond strengths achieved to be comparable to values obtained using conventional bonding methods. We also describe the use of carboxyl-terminated silanes on gold surfaces to bond amine-modified PDMS devices. Water contact angle measurements and X-ray photoelectron spectroscopy (XPS) confirmed the conjugation, a novel result that expands the variety of surface chemistries available for such bonding.
我们提出了一种新的用于聚二甲基硅氧烷（PDMS）基器件的键合技术，该技术在室温下采用化学表面改性。PDMS 表面被官能化以呈现伯胺基团，而玻璃或金基底被官能化以呈现羧酸基团。通过将两个表面接触并在室温下反应，在基底之间形成肽键，从而实现不可逆键合。剪切测试表明，所获得的键合强度与使用传统键合方法获得的值相当。我们还描述了在金表面上使用羧基封端的硅烷来键合胺修饰的 PDMS 器件。水接触角测量和 X 射线光电子能谱（XPS）证实了共轭，这是一个新颖的结果，扩展了可用于这种键合的表面化学的种类。
