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研究活性包装抗菌保护机制的新方法。

New approach to study the mechanism of antimicrobial protection of an active packaging.

机构信息

Department of Analytical Chemistry, Aragon Institute of Engineering Research (I3A), CPS, University of Zaragoza, Zaragoza, Spain.

出版信息

Foodborne Pathog Dis. 2010 Sep;7(9):1063-9. doi: 10.1089/fpd.2009.0516.

DOI:10.1089/fpd.2009.0516
PMID:20575672
Abstract

This article reports on the antimicrobial efficiency of a new active packaging concept based on the use of two essential oils (cinnamon and oregano) and their chemical descriptors (cinnamaldehyde, thymol, and carvacrol) against the Gram-positive bacterium Listeria monocytogenes, the Gram-negative bacterium Salmonella choleraesuis, the yeast Candida albicans, and the mold Aspergillus flavus. Complete inhibition of these microorganisms with either bactericidal or bacteriostatic effect has been demonstrated. It has been proven that the inhibition provided by these solutions is related not to the total amount of the active chemical released but to the amount of active compounds that reach the agar surface at a critical time. This critical time is notably related with the duration of the lag phase, as demonstrated for the bacteria, and shows that kinetic behavior has a critical role in the antimicrobial properties of the active packaging. Two different active films, polypropylene and the complex polyethylene-ethylenvynil alcohol, have been studied and a higher efficiency was found for polypropylene, mainly because of the mentioned kinetic reasons. These results can be used to understand the mechanism of action of the chemicals and provide valuable data for the development of the active packaging concept.

摘要

这篇文章报道了一种新的基于使用两种精油(肉桂和牛至)及其化学描述符(肉桂醛、百里香酚和香芹酚)的主动包装概念的抗菌效率,针对革兰氏阳性菌李斯特菌、革兰氏阴性菌肠炎沙门氏菌、酵母白色念珠菌和霉菌黄曲霉。已经证明这些微生物具有杀菌或抑菌作用的完全抑制。已经证明,这些溶液提供的抑制作用不是与释放的总活性化学物质的量有关,而是与在关键时间到达琼脂表面的活性化合物的量有关。这个关键时间与细菌的滞后期的持续时间显著相关,这表明动力学行为在主动包装的抗菌性能中起着关键作用。已经研究了两种不同的活性膜,聚丙烯和复杂的聚乙烯-乙烯醇,并且发现聚丙烯的效率更高,主要是由于上述动力学原因。这些结果可用于了解化学物质的作用机制,并为主动包装概念的开发提供有价值的数据。

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