Animal Health Service, P.O. Box 9, 7400 AA Deventer, The Netherlands.
Vet Microbiol. 2011 Jan 10;147(1-2):186-9. doi: 10.1016/j.vetmic.2010.05.041. Epub 2010 Jun 1.
In cattle, Coxiella burnetii infections are generally asymptomatic but can also be associated with reproductive disorders. Metritis is considered as one of the symptoms of C. burnetii infections, but reliable information is lacking. Therefore, information on the presence of C. burnetii in the uterine content of cows with metritis is important to increase our knowledge on this pathogen. In this study, the uterine content of 45 dairy cows with metritis belonging to 12 herds was tested for C. burnetii with a real-time PCR assay. Only one uterine sample tested PCR (highly) positive, all other samples were PCR negative. The PCR positive cow tested also positive for antibodies. Three other cows from other herds tested antibody positive. The bulk milk (BM) samples of these 12 herds were tested by real-time PCR assay and antibody-ELISA. Six BM samples (50%) were positive in PCR and 10 (83%) were positive in ELISA. Culturing the uterus samples by bacteriology, the most frequently cultured bacteria were arcanobacterium (n=24), E. coli (n=16), other streptococci (n=10), Streptococcus uberis (n=8) and Streptococcus dysgalactiae (n=5). This study indicates that C. burnetii is not an important cause for metritis in dairy herds, although apparently C. burnetii was or had been present in most of these herds.
在牛中，感染柯克斯体通常无症状，但也可能与生殖障碍有关。子宫内膜炎被认为是柯克斯体感染的症状之一，但可靠信息缺乏。因此，了解患有子宫内膜炎的奶牛的子宫内容物中是否存在柯克斯体对于增加我们对该病原体的认识很重要。在这项研究中，使用实时 PCR 检测了来自 12 个牛群的 45 头患有子宫内膜炎的奶牛的子宫内容物中是否存在柯克斯体。只有一个子宫样本 PCR（高度）呈阳性，所有其他样本均为 PCR 阴性。PCR 阳性的奶牛也检测到了抗体。其他 3 头来自其他牛群的奶牛抗体检测呈阳性。对这 12 个牛群的乳样进行了实时 PCR 检测和抗体 ELISA 检测。6 个乳样（50%）PCR 阳性，10 个（83%）ELISA 阳性。通过细菌培养子宫样本，最常培养的细菌是隐球菌（n=24）、大肠杆菌（n=16）、其他链球菌（n=10）、乳房链球菌（n=8）和无乳链球菌（n=5）。本研究表明，柯克斯体不是奶牛群子宫内膜炎的重要原因，尽管显然在大多数牛群中存在或曾经存在柯克斯体。
