Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
Vector Borne Zoonotic Dis. 2012 Aug;12(8):650-3. doi: 10.1089/vbz.2011.0953. Epub 2012 May 31.
Q fever is an important zoonotic disease caused by Coxiella burnetii. There are few reliable data about C. burnetii infection available. The aim of this study was to assess the importance and potential infectious sources of Q fever in Hungary. A total of 215 milk samples (10 individual samples from each herd and 1 bulk tank milk sample from each cattle herd), and 400 serum samples (20 from each herd) were tested from 15 dairy cattle herds and 5 sheep flocks located in different parts of Hungary. The study found 19.3% (58/300) and 38.0% (57/150) seropositivity in cattle, and 0% (0/100) and 6.0% (3/50) seropositivity in sheep, by complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA), respectively. C. burnetii DNA was detected by IS1111 element-based TaqMan real-time polymerase chain reaction (PCR) in 8.7% (13/150) of individual dairy cow milk samples, 4.0% (2/50) of individual sheep milk samples, and 66.7% (10/15) of dairy bulk tank milk samples. Samples taken from nine different commercially-available pasteurized cow milk products from different Hungarian producers were also tested for the presence of C. burnetii DNA, and eight of these samples were found to be positive (88.9%). The real-time PCR examination of 5402 ixodid ticks collected from different parts of the country yielded negative results. Knowledge of the true prevalence of Q fever is crucial for policymakers involved in evidence-based decision making.
Q 热是由贝氏柯克斯体引起的重要动物源性传染病。目前关于贝氏柯克斯体感染的可靠数据较少。本研究旨在评估 Q 热在匈牙利的重要性和潜在感染源。从分布在匈牙利不同地区的 15 个奶牛场和 5 个绵羊群中采集了 215 份牛奶样本（每个牛场采集 10 份个体样本和 1 份牛群混合奶样，每个绵羊群采集 10 份个体奶样）和 400 份血清样本（每个牛场采集 20 份）。通过补体结合试验（CFT）和酶联免疫吸附试验（ELISA）检测发现，奶牛的血清阳性率分别为 19.3%（58/300）和 38.0%（57/150），绵羊的血清阳性率分别为 0%（0/100）和 6.0%（3/50）。通过基于 IS1111 元件的 TaqMan 实时聚合酶链反应（PCR）检测发现，150 份个体奶牛奶样中有 8.7%（13/150）、50 份个体绵羊奶样中有 4.0%（2/50）和 15 个牛群混合奶样中有 66.7%（10/15）检测出贝氏柯克斯体 DNA。还对来自不同匈牙利生产商的 9 种市售巴氏消毒牛奶产品中的 C. burnetii DNA 进行了检测，其中 8 种呈阳性（88.9%）。对从该国不同地区采集的 5402 只蜱进行的实时 PCR 检测结果均为阴性。了解 Q 热的真实流行率对于参与循证决策的决策者至关重要。
