Vodicka P, Hemminki K
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
Sci Total Environ. 1991 Jan 1;101(1-2):121-30. doi: 10.1016/0048-9697(91)90110-z.
DNA-adduct formation, depurination and imidazole ringopening were followed in vitro using styrene oxide, ethyleneimine and dimethyl sulfate. Depurination was found to be about 50 times faster in nucleosides than in double-stranded DNA. The half-lives of depurination in DNA were 3 times faster for 7-(2-aminoethyl)guanine as compared to 7-methyl- and 7-(2-hydroxy-2-phenylethyl)deoxyguanosine. In neutrally 7-methylguanine was released some 60 times faster than that of guanine and adenine. This apparent discrepancy in depurination between alkylated and intact bases suggests the possibility of developing a sensitive method for monitoring of DNA alkylations formed by electrophillic chemicals, which might be based on labelling of apurinic sites and utilized for in vivo studies as well.
使用环氧苯乙烷、乙撑亚胺和硫酸二甲酯在体外跟踪DNA加合物的形成、脱嘌呤作用和咪唑环开环。发现核苷中的脱嘌呤作用比双链DNA中的快约50倍。与7-甲基-和7-(2-羟基-2-苯乙基)脱氧鸟苷相比，7-(2-氨基乙基)鸟嘌呤在DNA中的脱嘌呤半衰期快3倍。在中性条件下，7-甲基鸟嘌呤的释放速度比鸟嘌呤和腺嘌呤快约60倍。烷基化碱基与完整碱基在脱嘌呤作用上的这种明显差异表明，有可能开发一种灵敏的方法来监测亲电化学物质形成的DNA烷基化，该方法可能基于对无嘌呤位点的标记，也可用于体内研究。
