Institute of Laboratory Diagnostics, Kaiser Franz Josef Hospital, Vienna, Austria.
Eur J Appl Physiol. 2010 Nov;110(4):695-701. doi: 10.1007/s00421-010-1532-5. Epub 2010 Jun 26.
The present study aimed to investigate the acute effects of a single bout of high-intensive strength training on the production of cell-free plasma DNA (cf-DNA), as well as on the degradation of purine nucleotides as assessed by the concentration of xanthine (XA) and hypoxanthine (HX) in urine and serum. Twelve trained weightlifters performed six sets of six lifting exercises with 90-95% of the one repetition maximum. Blood samples and urine were obtained 1 h before training, immediately after finishing the exercise session and following 2 h of recovery. Cf-DNA, HX, and XA (in serum) significantly increased (P < 0.05-P < 0.001) immediately after heavy lifting exercise when compared with baseline levels, and significantly decreased (P < 0.05-P < 0.001) after 2 h of recovery. These results indicate that, cf-DNA and oxypurines might be relevant biomarkers for cellular damage, mechanical, energetic, and/or ischemic stress in context with exercise.
本研究旨在探讨单次高强度力量训练对细胞游离血浆 DNA（cf-DNA）产生的急性影响，以及通过尿液和血清中黄嘌呤（XA）和次黄嘌呤（HX）的浓度评估嘌呤核苷酸降解的情况。12 名有训练经验的举重运动员进行了六组六次举重练习，强度为 90-95%的一次重复最大值。在训练前 1 小时、运动结束后立即以及恢复 2 小时后采集血样和尿液。与基线水平相比，cf-DNA、HX 和 XA（血清）在剧烈举重运动后即刻显著增加（P<0.05-P<0.001），并在恢复 2 小时后显著降低（P<0.05-P<0.001）。这些结果表明，cf-DNA 和氧化嘌呤可能是与运动相关的细胞损伤、力学、能量和/或缺血应激的相关生物标志物。
